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Morphological Transformations in the Magnetite Biomineralizing Protein Mms6 in Iron Solutions: A Small-Angle X-ray Scattering Study
Langmuir
  • Honghu Zhang, Iowa State University
  • Xunpei Liu, Iowa State University
  • Shuren Feng, Iowa State University
  • Wenjie Wang, Iowa State University
  • Klaus Schmidt-Rohr, Iowa State University
  • Mufit Akinc, Iowa State University
  • Marit Nilsen-Hamilton, Iowa State University
  • David Vaknin, Iowa State University
  • Surya K. Mallapragada, Iowa State University
Document Type
Article
Publication Version
Published Version
Publication Date
1-1-2015
DOI
10.1021/la5044377
Abstract
Magnetotactic bacteria that produce magnetic nanocrystals of uniform size and well-defined morphologies have inspired the use of biomineralization protein Mms6 to promote formation of uniform magnetic nanocrystals in vitro. Small angle X-ray scattering (SAXS) studies in physiological solutions reveal that Mms6 forms compact globular three-dimensional (3D) micelles (approximately 10 nm in diameter) that are, to a large extent, independent of concentration. In the presence of iron ions in the solutions, the general micellar morphology is preserved, however, with associations among micelles that are induced by iron ions. Compared with Mms6, the m2Mms6 mutant (with the sequence of hydroxyl/carboxyl containing residues in the C-terminal domain shuffled) exhibits subtle morphological changes in the presence of iron ions in solutions. The analysis of the SAXS data is consistent with a hierarchical core–corona micellar structure similar to that found in amphiphilic polymers. The addition of ferric and ferrous iron ions to the protein solution induces morphological changes in the micellar structure by transforming the 3D micelles into objects of reduced dimensionality of 2, with fractal-like characteristics (including Gaussian-chain-like) or, alternatively, platelet-like structures.
Comments

Reprinted (adapted) with permission from Langmuir, 2015, 31 (9), pp 2818–2825, DOI: 10.1021/la5044377. Copyright (2015) American Chemical Society.

Copyright Owner
American Chemical Society
Language
en
File Format
application/pdf
Citation Information
Honghu Zhang, Xunpei Liu, Shuren Feng, Wenjie Wang, et al.. "Morphological Transformations in the Magnetite Biomineralizing Protein Mms6 in Iron Solutions: A Small-Angle X-ray Scattering Study" Langmuir Vol. 31 Iss. 9 (2015) p. 2818 - 2825
Available at: http://works.bepress.com/marit-nilsen-hamilton/25/