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Integrated self-assembly of the Mms6 magnetosome protein to form an Iron-responsive structure
International Journal of Molecular Sciences
  • Shuren Feng, Iowa State University
  • Lijun Wang, Iowa State University
  • Pierre Palo, Ames National Laboratory
  • Xunpei Liu, Iowa State University
  • Surya K. Mallapragada, Iowa State University
  • Marit Nilsen-Hamilton, Iowa State University
Document Type
Article
Publication Date
1-1-2013
DOI
10.3390/ijms140714594
Abstract
A common feature of biomineralization proteins is their self-assembly to produce a surface consistent in size with the inorganic crystals that they produce. Mms6, a small protein of 60 amino acids from Magnetospirillum magneticum strain AMB-1 that promotes the in vitro growth of superparamagnetic magnetite nanocrystals, assembles in aqueous solution to form spherical micelles that could be visualized by TEM and AFM. The results reported here are consistent with the view that the N and C-terminal domains interact with each other within one polypeptide chain and across protein units in the assembly. From studies to determine the amino acid residues important for self-assembly, we identified the unique GL repeat in the N-terminal domain with additional contributions from amino acids in other positions, throughout the molecule. Analysis by CD spectroscopy identified a structural change in the iron-binding C-terminal domain in the presence of Fe3+. A change in the intrinsic fluorescence of tryptophan in the N-terminal domain showed that this structural change is transmitted through the protein. Thus, self-assembly of Mms6 involves an interlaced structure of intraand inter-molecularinteractions that results in a coordinated structural change in the protein assembly with iron binding.
Comments

This is an article from International Journal of Molecular Sciences 14 (2013): 14594, doi: 10.3390/ijms140714594

Rights
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
Copyright Owner
Feng et al.
Language
en
File Format
application/pdf
Citation Information
Shuren Feng, Lijun Wang, Pierre Palo, Xunpei Liu, et al.. "Integrated self-assembly of the Mms6 magnetosome protein to form an Iron-responsive structure" International Journal of Molecular Sciences Vol. 14 Iss. 7 (2013) p. 14594 - 14606
Available at: http://works.bepress.com/marit-nilsen-hamilton/19/