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Article
Electrochromatographic Characterization of Etched Chemically-Modified Capillaries with Small Synthetic Peptides
Journal of Chromatography A (2001)
  • Maria T. Matyska-Pesek, San Jose State University
  • J. J. Pesek, San Jose State University
  • R. I. Boysen
  • M. T.W. Hearn
Abstract

In this investigation, various capillary electrochromatographic (CEC) experiments have been employed to characterize the properties of etched, chemically-modified surfaces of open tubular capillary columns with peptides as solute probes and under conditions of variable voltage, temperature and solvent composition. The separation performance of etched capillaries with either n-octadecyl or liquid crystal moieties derived from a cholesterol phase bonded to the surface were compared. With the liquid crystal bonded species, interesting and significantly different variations in retention behavior of peptides are obtained compared to those observed with the corresponding n-octadecyl modified surfaces by changes in temperature, solvent composition and field strength. These peptide separations illustrate the usefulness of this CEC approach for practical applications, where both the retention characteristics of the charged analytes as well as the selectivity differences due to the surface properties of the etched chemically-modified surfaces of open tubular capillary columns can be rationally modulated. As in HPLC, appropriate choice of CEC experimental variables, including the chemical properties of the immobilized ligand(s), represents a powerful tool for optimizing resolution.

Disciplines
Publication Date
2001
Publisher Statement
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Citation Information
Maria T. Matyska-Pesek, J. J. Pesek, R. I. Boysen and M. T.W. Hearn. "Electrochromatographic Characterization of Etched Chemically-Modified Capillaries with Small Synthetic Peptides" Journal of Chromatography A Vol. 924 Iss. 1-2 (2001)
Available at: http://works.bepress.com/maria_matyska-pesek/69/