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Article
Retention of Proteins and Metalloproteins in Open Tubular Capillary Electrochromatography with Etched Chemically Modified Columns
Electrophoresis (2008)
  • J. J. Pesek, San Jose State University
  • Maria T. Matyska-Pesek, San Jose State University
  • V. Salgotra
Abstract

Etched chemically modified capillaries with two different bonded groups (pentyl and octadecyl) are compared for their migration behavior of several common proteins and metalloproteins as well as metalloproteinases. Migration times, efficiency and peak shape are evaluated over the pH range of 2.1–8.1 to determine any effects of the bonded group on the electrochromatographic behavior of these compounds. One goal was to determine if the relative hydrophobicity of the stationary phase has a significant effect on proteins in the open tubular format of capillary electrochromatography as it does in HPLC. Reproducibility of the migration times is also investigated.

Disciplines
Publication Date
2008
Publisher Statement
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Citation Information
J. J. Pesek, Maria T. Matyska-Pesek and V. Salgotra. "Retention of Proteins and Metalloproteins in Open Tubular Capillary Electrochromatography with Etched Chemically Modified Columns" Electrophoresis Vol. 29 Iss. 18 (2008)
Available at: http://works.bepress.com/maria_matyska-pesek/28/