Skip to main content
Article
Synthesis of a Preparative C30 Stationary Phase on a Silica Hydride Surface and its Application to Carotenoid Separation
Journal of Liquid Chromatography and Related Technologies (2011)
  • Joseph J. Pesek, San Jose State University
  • Maria T. Matyska-Pesek, San Jose State University
  • Paul Lee, San Jose State University
Abstract
A triacontyl (C30) stationary phase has been synthesized on a silica hydride surface for use in preparative applications. The success of bonding via hydrosilation was monitored by diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy and elemental (carbon) analysis. A semi-preparative column was packed in order to test this material for potential applications. The test compounds selected for this evaluation were carotenoids. A number of organic solvents applicable to either normal or reversed-phase phase retention were used as binary mobile phase components. The effect of flow rate on resolution was also determined.
Keywords
  • long chain alkyl phase,
  • normal phase separations,
  • silica hydrid
Disciplines
Publication Date
February 2, 2011
DOI
10.1080/10826076.2011.546178
Publisher Statement
SJSU users: use the following link to login and access the article via SJSU databases
Citation Information
Joseph J. Pesek, Maria T. Matyska-Pesek and Paul Lee. "Synthesis of a Preparative C30 Stationary Phase on a Silica Hydride Surface and its Application to Carotenoid Separation" Journal of Liquid Chromatography and Related Technologies Vol. 34 Iss. 3 (2011) p. 231 - 240 ISSN: 1082-6076
Available at: http://works.bepress.com/maria_matyska-pesek/13/