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Article
Synthesis of a Preparative C30 Stationary Phase on a Silica Hydride Surface and its Application to Carotenoid Separation
Journal of Liquid Chromatography and Related Technologies (2011)
  • J. J. Pesek, San Jose State University
  • Maria T. Matyska-Pesek, San Jose State University
  • P. Lee, San Jose State University
Abstract

A triacontyl (C30) stationary phase has been synthesized on a silica hydride surface for use in preparative applications. The success of bonding via hydrosilation was monitored by diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy and elemental (carbon) analysis. A semi-preparative column was packed in order to test this material for potential applications. The test compounds selected for this evaluation were carotenoids. A number of organic solvents applicable to either normal or reversed-phase phase retention were used as binary mobile phase components. The effect of flow rate on resolution was also determined.

Disciplines
Publication Date
2011
Publisher Statement
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Citation Information
J. J. Pesek, Maria T. Matyska-Pesek and P. Lee. "Synthesis of a Preparative C30 Stationary Phase on a Silica Hydride Surface and its Application to Carotenoid Separation" Journal of Liquid Chromatography and Related Technologies Vol. 34 Iss. 3 (2011)
Available at: http://works.bepress.com/maria_matyska-pesek/13/