Synthesis of a Preparative C30 Stationary Phase on a Silica Hydride Surface and its Application to Carotenoid SeparationJournal of Liquid Chromatography and Related Technologies (2011)
A triacontyl (C30) stationary phase has been synthesized on a silica hydride surface for use in preparative applications. The success of bonding via hydrosilation was monitored by diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy and elemental (carbon) analysis. A semi-preparative column was packed in order to test this material for potential applications. The test compounds selected for this evaluation were carotenoids. A number of organic solvents applicable to either normal or reversed-phase phase retention were used as binary mobile phase components. The effect of flow rate on resolution was also determined.
- long chain alkyl phase,
- normal phase separations,
- silica hydrid
Publication DateFebruary 2, 2011
Citation InformationJoseph J. Pesek, Maria T. Matyska-Pesek and Paul Lee. "Synthesis of a Preparative C30 Stationary Phase on a Silica Hydride Surface and its Application to Carotenoid Separation" Journal of Liquid Chromatography and Related Technologies Vol. 34 Iss. 3 (2011) p. 231 - 240 ISSN: 1082-6076
Available at: http://works.bepress.com/maria_matyska-pesek/13/