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Molecular imaging of cyclooxygenase-2 in canine transitional cell carcinomas in vitro and in vivo
Cancer Prevention Research (2013)
  • Maria Cekanova, MS, RNDr, PhD, University of Tennessee - Knoxville
  • M J Uddin, Vanderbilt University
  • Joseph W Bartges, University of Tennessee - Knoxville
  • Amanda Callens, University of Tennessee - Knoxville
  • Kusum Rathore, University of Tennessee - Knoxville
  • Alfred Legendre, University of Tennessee - Knoxville
  • L Wright, Vanderbilt University
  • L J Marnett, University of Tennessee - Knoxville
Abstract

The enzyme COX-2 is induced at high levels in tumors but not in surrounding normal tissues, which makes it an attractive target for molecular imaging of cancer. We evaluated the ability of novel optical imaging agent, fluorocoxib A to detect urinary bladder canine transitional cell carcinomas (K9TCC). Here, we show that fluorocoxib A uptake overlapped with COX-2 expression in primary K9TCC cells in vitro. Using subcutaneously implanted primary K9TCC in athymic mice, we show specific uptake of fluorocoxib A by COX-2-expressing K9TCC xenograft tumors in vivo. Fluorocoxib A uptake by COX-2-expressing xenograft tumors was blocked by 70% (P < 0.005) when pretreated with the COX-2 selective inhibitor, celecoxib (10 mg/kg), 4 hours before intravenous administration of fluorocoxib A (1 mg/kg). Fluorocoxib A was taken up by COX-2-expressing tumors but not by COX-2-negative human UMUC-3 xenograft tumors. UMUC-3 xenograft tumors with no expression of COX-2 showed no uptake of fluorocoxib A. In addition, fluorocoxib A uptake was evaluated in five dogs diagnosed with TCC. Fluorocoxib A specifically detected COX-2-expressing K9TCC during cystoscopy in vivo but was not detected in normal urothelium. Taken together, our findings show that fluorocoxib A selectively bound to COX-2-expressing primary K9TCC cells in vitro, COX-2-expressing K9TCC xenografts tumors in nude mice, and heterogeneous canine TCC during cystoscopy in vivo. Spontaneous cancers in companion animals offer a unique translational model for evaluation of novel imaging and therapeutic agents using primary cancer cells in vitro and in heterogeneous cancers in vivo.

Publication Date
May, 2013
Citation Information
Maria Cekanova, M J Uddin, Joseph W Bartges, Amanda Callens, et al.. "Molecular imaging of cyclooxygenase-2 in canine transitional cell carcinomas in vitro and in vivo" Cancer Prevention Research Vol. 6 Iss. 5 (2013)
Available at: http://works.bepress.com/maria_cekanova/29/