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Increase in ACC Oxidase Levels and Activities During Paradormancy Release of Leafy Spurge (Euphorbia Esula) Buds
Planta
  • Wun S. Chao, USDA-Agricultural Research Service
  • Marcelo Serpe, Boise State University
  • Jeffrey C. Suttle, USDA-Agricultural Research Service
  • Ying Jia, Texas A & M University - Kingsville
Document Type
Article
Publication Date
7-1-2013
Disciplines
Abstract

The plant hormone ethylene is known to affect various developmental processes including dormancy and growth. Yet, little information is available about the role of ethylene during paradormancy release in underground adventitious buds of leafy spurge. In this study, we examined changes in ethylene evolution and the ethylene biosynthetic enzyme ACC oxidase following paradormancy release (growth induction). Our results did not show an obvious increase in ethylene during bud growth. However, when buds were incubated with 1 mM ACC, ethylene levels were higher in growing than non-growing buds, suggesting that the levels of ACC oxidase increased in growing buds. Real-time qPCR indicated that the transcript of a Euphorbia esula ACC oxidase (Ee-ACO) increased up to threefold following growth induction. In addition, a 2.5- to 4-fold increase in ACO activity was observed 4 days after decapitation, and the Ee-ACO accounted for 40 % of the total ACO activity. Immunoblot analyses identified a 36-kD Ee-ACO protein that increased in expression during bud growth. This protein was highly expressed in leaves, moderately expressed in crown buds, stems and meristems, and weakly expressed in roots and flowers. Immunolocalization of Ee-ACO on growing bud sections revealed strong labeling of the nucleus and cytoplasm in cells at the shoot apical meristem and leaf primordia. An exception to this pattern occurred in cells undergoing mitosis, where labeling of Ee-ACO was negligible. Taken together, our results indicated an increase in the levels of Ee-ACO during paradormancy release of leafy spurge that was not correlated with an increase in ethylene synthesis.

Copyright Statement

This is an author-produced, peer-reviewed version of this article. The final publication is available at www.springerlink.com. Copyright restrictions may apply. DOI: 10.1007/s00425-013-1887-z.

This is an author-produced, peer-reviewed version of this article. The final publication is available at www.springerlink.com. Copyright restrictions may apply. DOI: [DOI #]

Citation Information
Wun S. Chao, Marcelo Serpe, Jeffrey C. Suttle and Ying Jia. "Increase in ACC Oxidase Levels and Activities During Paradormancy Release of Leafy Spurge (Euphorbia Esula) Buds" Planta (2013)
Available at: http://works.bepress.com/marcelo_serpe/20/