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EGCG Protects against 6-OHDA-Induced Neurotoxicity in a Cell Culture Model
Parkinson’s Disease
  • Dan Chen, Iowa State University
  • Anumantha G. Kanthasamy, Iowa State University
  • Manju B. Reddy, Iowa State University
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Background. Parkinson’s disease (PD) is a progressive neurodegenerative disease that causes severe brain dopamine depletion. Disruption of iron metabolism may be involved in the PD progression. Objective. To test the protective effect of (−)-epigallocatechin-3-gallate (EGCG) against 6-hydroxydopamine- (6-OHDA-) induced neurotoxicity by regulating iron metabolism in N27 cells. Methods. Protection by EGCG in N27 cells was assessed by SYTOX green assay, MTT, and caspase-3 activity. Iron regulatory gene and protein expression were measured by RT-PCR and Western blotting. Intracellular iron uptake was measured using 55Fe. The EGCG protection was further tested in primary mesencephalic dopaminergic neurons by immunocytochemistry. Results. EGCG protected against 6-OHDA-induced cell toxicity. 6-OHDA treatment significantly () increased divalent metal transporter-1 (DMT1) and hepcidin and decreased ferroportin 1 (Fpn1) level, whereas pretreatment with EGCG counteracted the effects. The increased 55Fe (by 96%, ) cell uptake confirmed the iron burden by 6-OHDA and was reduced by EGCG by 27% (), supporting the DMT1 results. Pretreatment with EGCG and 6-OHDA significantly increased () TH+ cell count (~3-fold) and neurite length (~12-fold) compared to 6-OHDA alone in primary mesencephalic neurons. Conclusions. Pretreatment with EGCG protected against 6-OHDA-induced neurotoxicity by regulating genes and proteins involved in brain iron homeostasis, especially modulating hepcidin levels.

This article is published as Chen D, Kanthasamy AG, Reddy MB. EGCG protects against 6-OHDA induced neurotoxicity in a cell culture model. Parkinson’s Disease, 2015 (doi:10.1155/2015/843906)

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Dan Chen, Anumantha G. Kanthasamy and Manju B. Reddy. "EGCG Protects against 6-OHDA-Induced Neurotoxicity in a Cell Culture Model" Parkinson’s Disease Vol. 843906 (2015)
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