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Article
Quantification of Ferritin from Staple Food Crops
Journal of Agricultural and Food Chemistry
Document Type
Article
Disciplines
Publication Date
3-25-2009
DOI
10.1021/jf803381d
Abstract
Ferritin-iron has been shown to be as bioavailable as ferrous sulfate in humans. Thus, biofortification to breed crops with high ferritin content is a promising strategy to alleviate the global iron deficiency problem. Although ferritin is present in all food crops, its concentration varies between species and varieties. Therefore, a successful ferritin biofortification strategy requires a method to rapidly measure ferritin concentrations in food crops. The objective of this study was to develop a simple and reliable ELISA using an anti-ferritin polyclonal antibody to detect ferritin in various crops. Crude seed extracts were found to have 10.2 +/- 1.0, 4.38 +/- 0.9, 1.2 +/- 0.3, 0.38 +/- 0.1, and 0.04 +/- 0.01 microg of ferritin/g of dry seed in red beans, white beans, wheat, maize, and brown rice, respectively. Although the measured absolute concentrations of ferritin values were low, the presented method is applicable for rapid screening for the relative ferritin concentrations of large numbers of seeds to identify and breed ferritin-rich crops.
Copyright Owner
American Chemical Society
Copyright Date
2009
Language
en
File Format
application/pdf
Citation Information
Rebbecca J. Lukac, Maneesha R. Aluru and Manju B. Reddy. "Quantification of Ferritin from Staple Food Crops" Journal of Agricultural and Food Chemistry Vol. 57 Iss. 6 (2009) p. 2155 - 2161 Available at: http://works.bepress.com/manju_reddy/25/
Reprinted with permission from Journal of Agricultural and Food Chemistry 2009, 57(6): 2155. doi:10.1021/jf803381d . Copyright 2009 American Chemical Society.