Skip to main content
Article
Identification of the Larval and Juvenile Stages of the Cubera Snapper, Lutjanus cyanopterus, Using DNA Barcoding
Zootaxa
  • Benjamin C. Victor, Ocean Science Foundation, Nova Southeastern University
  • Robert Hanner, University of Guelph
  • Mahmood S. Shivji, Nova Southeastern University
  • John Hyde, Southwest Fisheries Science Center
  • Chris Caldow, National Oceanic and Atmospheric Association
Document Type
Article
Publication Date
8-31-2009
Keywords
  • Snapper,
  • Cubera,
  • Lutjanidae,
  • Lutjanus,
  • cyanopterus,
  • larvae,
  • juvenile,
  • identification,
  • DNA barcoding,
  • BOLD,
  • fish,
  • phylogenetics,
  • pelagic larval duration,
  • PLD,
  • pargo,
  • gray,
  • griseus,
  • Caribbean,
  • western Atlantic,
  • otoliths
Peer Reviewed
1
Abstract
The larvae and newly-settled juveniles of the Cubera Snapper, Lutjanus cyanopterus, are identified by DNA barcoding. Four larvae and three small juveniles of L. cyanopterus were detected among a large collection of pelagic larvae and a smaller collection of settled juveniles from the Caribbean coast of Panama. The mtDNA COI barcode sequences from the larvae and juveniles were virtually identical to sequences from adults sampled from the spawning aggregation in St. Thomas, USVI. Barcode sequences for the eleven regional species of Lutjanus species (sensu lato) were obtained and they exhibited deep interspecific divergences that allowed for efficient discrimination among the western Atlantic snapper species. The nearest neighbor species, the Mutton Snapper L. analis, was more than 11% divergent from L. cyanopterus. Cubera Snapper larvae are characterized by prominent melanophores along the outer spinous-dorsal-fin membranes and along the outer third of the longer pelvic-fin membranes. They are morphologically distinct from the late-stage larvae of the other regional snappers by their relatively wider caudal peduncle and their relative dorsal-spine lengths. Juveniles retain the black outer portion of the dorsal and pelvic-fin membranes and have a smaller body-depth than other regional snapper juveniles. The size at settlement is about 18 mm SL, relatively large for lutjanids. Daily otolith increments from L. cyanopterus larvae and juveniles indicate a pelagic larval duration of about 29 days with backcalculated spawning and settlement dates around the new moon. Although smaller adult Cubera Snappers can appear very similar to the Gray Snapper, L. griseus, the larvae and juveniles are quite different. In this case, the early life history stages reflect the deep genetic divergence between the two species while the adult forms converge in appearance.
Comments

Copyright © 2009 · Magnolia Press

Additional Comments
DNA barcoding was supported through funding to the Canadian Barcode of Life Network from Genome Canada (through the Ontario Genomics Institute), NSERC and other sponsors listed at www.BOLNET.ca.
ResearcherID
G-4080-2013
Citation Information
Benjamin C. Victor, Robert Hanner, Mahmood S. Shivji, John Hyde, et al.. "Identification of the Larval and Juvenile Stages of the Cubera Snapper, Lutjanus cyanopterus, Using DNA Barcoding" Zootaxa Vol. 2215 (2009) p. 24 - 36 ISSN: 1175-5326
Available at: http://works.bepress.com/mahmood-shivji/70/