Skip to main content
Article
Development and Characterization of Fifteen Novel Microsatellite Loci for the Nassau Grouper (Epinephelus striatus) and Their Utility for Cross-Amplification on a Suite of Closely Related Species
Conservation Genetics Resources
  • Andrea M. Bernard, Nova Southeastern University
  • Kevin A. Feldheim, Pritzker Laboratory for Molecular Systematics and Evolution
  • Vincent P. Richards, Nova Southeastern University, Cornell University
  • Richard S. Nemeth, University of the Virgin Islands
  • Mahmood S. Shivji, Nova Southeastern University
Document Type
Article
Publication Date
12-1-2012
Keywords
  • Grouper genetics,
  • Grouper conservation,
  • Serranidae,
  • Epinephelus,
  • Mycteroperca
Peer Reviewed
0
Abstract

The Nassau grouper (Epinephelus striatus), a severely overexploited and endangered coral reef fish in the Western Atlantic, is in urgent need of conservation and management measures. We report the development and characterization of 15 polymorphic, mostly tetranucleotide microsatellite loci based on 40 Nassau grouper samples collected from two Caribbean spawning aggregations. The number of alleles per locus and expected heterozygosities ranged from 4 to 19 and 0.40 to 0.92, respectively. We also demonstrate the cross-amplification utility of these microsatellites for genetic studies of five other grouper species of conservation interest.

Comments

©Springer Science+Business Media B.V. 2012

Additional Comments
US Geological Survey Project #: 07ERAG0078; NOAA Center for Sponsored Coastal Ocean Science award #: NA10NOS4260221
ResearcherID
G-4080-2013
DOI
10.1007/s12686-012-9688-4
Citation Information
Andrea M. Bernard, Kevin A. Feldheim, Vincent P. Richards, Richard S. Nemeth, et al.. "Development and Characterization of Fifteen Novel Microsatellite Loci for the Nassau Grouper (Epinephelus striatus) and Their Utility for Cross-Amplification on a Suite of Closely Related Species" Conservation Genetics Resources Vol. 4 Iss. 4 (2012) p. 983 - 986 ISSN: 1877-7252
Available at: http://works.bepress.com/mahmood-shivji/45/