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Article
Development and Characterization of Thirteen Microsatellite Markers for the Longbill Spearfish (Tetrapturus pfluegeri)
Conservation Genetics Resources
  • Andrea M. Bernard, Nova Southeastern University
  • A. W. S. Hilsdorf, Universidade de Mogi das Cruzes - Brazil
  • A. F. Amorim, Agencia Paulista de Tecnologia dos Agronegocios - Brazil
  • Rodrigo Rodrigues Domingues, Universidade de Mogi das Cruzes - Brazil
  • Mahmood S. Shivji, Nova Southeastern University
Document Type
Article
Publication Date
9-28-2013
Keywords
  • Billfish genetics,
  • Billfish conservation and management,
  • Istiophoridae,
  • STR
Peer Reviewed
0
Abstract

The longbill spearfish (Tetrapturus pfluegeri; Istiophoridae) is an epipelagic billfish species broadly distributed in the Atlantic Ocean and exploited by multinational fisheries. No data exist on the population structure or genetic diversity of this apex predator, despite a strong need for this information to inform international conservation and management efforts. Thirteen microsatellite loci, comprising mostly tri- and tetra-nucleotide repeats, were isolated and characterized for this species by genotyping individuals (n = 29–42) obtained from western North and South Atlantic fisheries. The average number of alleles ranged from 3 to 16 and the expected heterozygosity ranged from 0.35 to 0.86. This suite of markers provides the first population genetic nuclear resources for the longbill spearfish, allowing initiation of studies for assessing the population structure and demography of this poorly understood species.

Comments

©Springer Science+Business Media Dordrecht 2013

Additional Comments
FAPESP grant #: 2009/54.660-6
ResearcherID
G-4080-2013
DOI
10.1007/s12686-013-0038-y
Citation Information
Andrea M. Bernard, A. W. S. Hilsdorf, A. F. Amorim, Rodrigo Rodrigues Domingues, et al.. "Development and Characterization of Thirteen Microsatellite Markers for the Longbill Spearfish (Tetrapturus pfluegeri)" Conservation Genetics Resources Vol. 6 Iss. 1 (2013) p. 169 - 171 ISSN: 1877-7252
Available at: http://works.bepress.com/mahmood-shivji/41/