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Article
Development and Characterization of 11 Novel Microsatellite Loci for the Roundscale Spearfish Tetrapturus georgii and Their Cross-Species Amplification Among Other Istiophorid Species
Journal of Fish Biology
  • Andrea M. Bernard, Nova Southeastern University
  • Kevin A. Feldheim, Pritzker Laboratory for Molecular Systematics and Evolution
  • Mahmood S. Shivji, Nova Southeastern University
Document Type
Article
Publication Date
9-14-2012
Keywords
  • Atlantic,
  • billfish,
  • Istiophoridae,
  • population genetics,
  • primer sequence
Peer Reviewed
1
Abstract
Eleven novel polymorphic microsatellite loci were developed and characterized for the recently validated roundscale spearfish Tetrapturus georgii. Characterization of these markers, based on 35 roundscale spearfish from the western North Atlantic, revealed two to 21 alleles per locus with an average expected heterozygosity (HE) of 0·09–0·94, and all loci conformed to Hardy–Weinberg expectations. Cross-amplification of these 11 loci against all other eight known istiophorid species indicates promising prospects for the utility of these markers for istiophorids in general.
Comments

© 2012 The Authors

ResearcherID
G-4080-2013
DOI
10.1111/j.1095-8649.2012.03442.x
Citation Information
Andrea M. Bernard, Kevin A. Feldheim and Mahmood S. Shivji. "Development and Characterization of 11 Novel Microsatellite Loci for the Roundscale Spearfish Tetrapturus georgii and Their Cross-Species Amplification Among Other Istiophorid Species" Journal of Fish Biology Vol. 81 Iss. 5 (2012) p. 1781 - 1786 ISSN: 0022-1112
Available at: http://works.bepress.com/mahmood-shivji/39/