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Genomes of endangered great hammerhead and shortfin mako sharks reveal historic population declines and high levels of inbreeding in great hammerhead
iScience
  • Michael J. Stanhope, Cornell University
  • Kristina M. Ceres, Cornell University
  • Qi Sun, Cornell University
  • Minghui Wang, Cornell University
  • Jordan D. Zehr, Temple University
  • Nicholas J. Marra, Governors State University
  • Aryn P. Wilder, San Diego Zoo Wildlife Alliance
  • Cheng Zou, Cornell University
  • Andrea M Bernard, Nova Southeastern University
  • Paulina D. Pavinski Bitar, Cornell University
  • Mitchell G. Lokey, Cornell University
  • Mahmood Shivji, Nova Southeastern University
Document Type
Article
Publication Date
1-20-2023
Abstract

Despite increasing threats of extinction to Elasmobranchii (sharks and rays), whole genome-based conservation insights are lacking. Here, we present chromosome-level genome assemblies for the Critically Endangered great hammerhead (Sphyrna mokarran) and the Endangered shortfin mako (Isurus oxyrinchus) sharks, with genetic diversity and historical demographic comparisons to other shark species. The great hammerhead exhibited low genetic variation, with 8.7% of the 2.77 Gbp genome in runs of homozygosity (ROH) > 1 Mbp and 74.4% in ROH >100 kbp. The 4.98 Gbp shortfin mako genome had considerably greater diversity and 1 Mbp. Both these sharks experienced precipitous declines in effective population size (Ne) over the last 250 thousand years. While shortfin mako exhibited a large historical Ne that may have enabled the retention of higher genetic variation, the genomic data suggest a possibly more concerning picture for the great hammerhead, and a need for evaluation with additional individuals.

Comments
Data and code availability
  • Genome assembly data for the great hammerhead and shortfin mako have been deposited at GenBank and are publicly available as of the date of publication. The genome sequencing reads and transcriptome sequence data are deposited in the NCBI Sequence Read Archive. Accession numbers are listed in the key resources table.
  • Preliminary genome annotations for both the great hammerhead and shortfin mako have been deposited at Dryad and are publicly available as of the date of publication. DOIs are listed in the key resources table.

  • Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.

Additional Comments
Major funding for this study was provided by grants from the Save Our Seas Foundation, the Guy Harvey Ocean Foundation, and the Shark Foundation/Hai Stiftung.
Creative Commons License
Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International
ResearcherID
G-4080-2013
DOI
10.1016/j.isci.2022.105815
Citation Information
Michael J. Stanhope, Kristina M. Ceres, Qi Sun, Minghui Wang, et al.. "Genomes of endangered great hammerhead and shortfin mako sharks reveal historic population declines and high levels of inbreeding in great hammerhead" iScience Vol. 26 Iss. 1 (2023) ISSN: 2589-0042
Available at: http://works.bepress.com/mahmood-shivji/230/