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Article
Genetic Connectivity of a Coral Reef Ecosystem Predator: The Population Genetic Structure and Evolutionary History of the Caribbean Reef Shark (Carcharhinus perezi)
Journal of Biogeography
  • Andrea Bernard, Nova Southeastern University
  • Rebekah L. Horn, Nova Southeastern University
  • Demian D. Chapman, State University of New York - Stony Brook
  • Kevin A. Feldheim, Field Museum of Natural History - Chicago
  • Ricardo C. Garla, Universidade Federal do Rio Grande do Norte - Brazil
  • Edd J. Brooks, Cape Eleuthera Institute - Bahamas, United Kingdom
  • Mauvis A. Gore, Marine Conservation International - South Queensferry, United Kingdom
  • Mahmood S. Shivji, Nova Southeastern University
Document Type
Article
Publication Date
7-26-2017
Keywords
  • Coral reefs,
  • Elasmobranch,
  • Evolutionary history,
  • Marine biogeography,
  • Microsatellite DNA,
  • Mitochondrial DNA,
  • Population structure,
  • Western Atlantic
Abstract
Aim The Caribbean reef shark (Carcharhinus perezi) is one of few extant reef sharks inhabiting the Atlantic Ocean. Its variability in movements across habitat types suggests the possibility of a complex genetic population structure. Here, we use mitochondrial and nuclear DNA to investigate the genetic connectivity of the Caribbean reef shark across contemporary and evolutionary time-scales and relate our findings to the ecology of this understudied species. Location Tropical western Atlantic and Caribbean. Methods Samples were obtained from 216 individuals from six western Atlantic and Caribbean locations. Individuals were genotyped at seven nuclear microsatellite DNA loci and sequenced at two mitochondrial (control region [CR]; NADH dehydrogenase subunit 4 [ND4]) and one nuclear locus (lactate dehydrogenase [LDH]). Analyses to resolve the population genetic structure and evolutionary history of this species were adopted. Results Sequencing of the CR (1,068 bp, n = 216), ND4 (741 bp, n = 213) and LDH (258 bp, n = 165) loci, resolved 11, 8 and 13 unique haplotypes (or alleles), respectively. Overall, Caribbean reef sharks showed low levels of genetic diversity and most marker sets identified strong genetic differences (FSTand ΦST) between sharks sampled in Brazil versus all other locations (msat FST > 0.017; CR-ND4 ΦST > 0.013). Mitochondrial DNA showed evidence of increased genetic partitioning among western North Atlantic sampling sites, although widespread haplotype sharing (~85%–92%) and a shallow population history were found. Main Conclusions Findings of genetic differentiation are concordant with previous movement studies showing residency and/or site-fidelity to specific locations by individuals. However, similar to other reef shark studies, we found that the level of genetic connectivity among populations was context dependent—i.e., sharks occupying isolated habitats showed greater genetic differentiation compared with those sharks occupying semi-isolated or continuous reef habitats. Furthermore, low genetic diversity and a shallow mitochondrial population history were found, suggesting historical demographic fluctuations, including population collapse and more recent expansions.
Comments

©2017 John Wiley & Sons Ltd

Additional Comments
Sao Paulo Research Foundation grant #: FAPESP #1998/15080-8
ResearcherID
G-4080-2013
DOI
10.1111/jbi.13062
Citation Information
Andrea Bernard, Rebekah L. Horn, Demian D. Chapman, Kevin A. Feldheim, et al.. "Genetic Connectivity of a Coral Reef Ecosystem Predator: The Population Genetic Structure and Evolutionary History of the Caribbean Reef Shark (Carcharhinus perezi)" Journal of Biogeography (2017) p. 1 - 13 ISSN: 0305-0270
Available at: http://works.bepress.com/mahmood-shivji/137/