This study denotes a threshold to utilize Guayule Resin as a new bio-based binder or asphalt cement (AC) proportion, both with/out crumb rubber modifier (CRM), regarding solutions for environment, cost, and sustainability aspects. Via this research, 15 designated binders will be presented, all of them were tested as unaged and RTFO, whereas eight binders proceeded with PAV. Superpave was utilized to study their physical properties. Viscosity was examined upon some guayule-based binders with/out CRM, getting 114cP (at 135℃) for BGR, increased to about 3 times with 10%CRM (by weight of liquid binder) and about 7-8 times with 20%CRM. Comparisons among binders will be depicted regarding six categories (Neat AC, Neat BGR, AC+BGR, BGR+CRM, AC+CRM, and AC+BGR+CRM). This study initiates a perspective to judge designated binders and get benefit upon performance required. That's why the study reported outcomes regarding the effectiveness of the binder's stiffness, elasticity and performance. Results showed 20%CRM raised BGR performance by about one grade, compared to 5%CRM for AC to boost the same one grade. On another side, RTFOT showed a high mass loss for guayule-based binders, reaching 8.5% for unconditioned BGR, but about 5% by a heat-treatment process. The low-temperature grade was presented for all guayule-based binders indicating a-10 grade.