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Lifeact-mEGFP Reveals a Dynamic Apical F-Actin Network in Tip Growing Plant Cells
PLoS ONE (2009)
  • L Vidali
  • CM Rounds
  • PK Hepler
  • M Bezanilla


Actin is essential for tip growth in plants. However, imaging actin in live plant cells has heretofore presented challenges. In previous studies, fluorescent probes derived from actin-binding proteins often alter growth, cause actin bundling and fail to resolve actin microfilaments.

Methodology/Principal Findings

In this report we use Lifeact-mEGFP, an actin probe that does not affect the dynamics of actin, to visualize actin in the moss Physcomitrella patens and pollen tubes from Lilium formosanum and Nicotiana tobaccum. Lifeact-mEGFP robustly labels actin microfilaments, particularly in the apex, in both moss protonemata and pollen tubes. Lifeact-mEGFP also labels filamentous actin structures in other moss cell types, including cells of the gametophore.


Lifeact-mEGFP, when expressed at optimal levels does not alter moss protonemal or pollen tube growth. We suggest that Lifeact-mEGFP represents an exciting new versatile probe for further studies of actin's role in tip growing plant cells.

Publication Date
January 1, 2009
Publisher Statement
Attribution CC BY
Citation Information
L Vidali, CM Rounds, PK Hepler and M Bezanilla. "Lifeact-mEGFP Reveals a Dynamic Apical F-Actin Network in Tip Growing Plant Cells" PLoS ONE Vol. 4 Iss. 5 (2009)
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