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Proliferation, osteogenic and chrondrogenic differentiation of equine-derived bone marrow mesenchymal stem cells on bacterial cellulose scaffolds
The FASEB Journal (2012)
  • Pelagie M Favi
  • Christopher Stephens
  • Nancy Neilsen
  • Ryan Hammonds
  • Roberto Benson
  • Madhu S Dhar, University of Tennessee, Knoxville
Abstract
The purpose of this study was to evaluate the cell proliferation and viability, osteocyte and chondrocyte differentiation of equine-derived bone marrow mesenchymal stem cells (Eq-MSCs) when seeded onto biocompatible bacterial cellulose (BC) scaffolds. BC was synthesized using the bacterium Gluconacetobacter sucrofermentans. Previously isolated, expanded and characterized Eq-MSCs were seeded on the BC scaffolds and fluorescence microscopy confirmed adherence and expansion. Confocal imaging confirmed the cells positively stained for CD90 antibody, a common surface marker for mesenchymal stem cells. MTS assay demonstrated increasing proliferation and viability with time (days 2, 7 and14). Cell-scaffold constructs were cultured for 14 days under osteogenic and chondrogenic conditions. Osteocytes were positive for alizarin red and chondrogenesis was confirmed by alcian blue staining of the chondrocytes. In summary, biocompatible BC scaffolds support the proliferation and viability, osteogenic and chondrogenic differentiation of Eq-MSCs cultured onto its surface in-vitro, allowing for future potential use for tissue engineering therapies. This research is sponsored by the Physicians Medical and Education Research Foundation Grant, the Cardiothoracic Surgery Gift Fund, and the National Institute of Health Training Grant.
Disciplines
Publication Date
2012
Citation Information
Pelagie M Favi, Christopher Stephens, Nancy Neilsen, Ryan Hammonds, et al.. "Proliferation, osteogenic and chrondrogenic differentiation of equine-derived bone marrow mesenchymal stem cells on bacterial cellulose scaffolds" The FASEB Journal Vol. 26 (2012)
Available at: http://works.bepress.com/madhu_dhar/46/