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Sequence of a cDNA Encoding the Ferritin h-chain from an 11-week-old Human Fetal Brain
Gene (1993)
  • Madhu S Dhar, University of Tennessee, Knoxville
  • V. Chauthaiwale, University of Tennessee, Knoxville
  • J. G. Joshi, University of Tennessee, Knoxville
A cDNA library in λ Charon BS(−) from 11-week-old human fetal brain (FB) was screened using a human liver ferritin (FTH)-encoding cDNA as a probe. The complete sequence of the positive clone, cFB1, showed that the coding region and a part of the 5′ and 3′ untranslated regions (UTR) are identical to the corresponding published sequence of the liver cDNA. However, a particularly noteworthy difference is the presence of 279 bp of additional sequence in the FB 3′-UTR. Northern blot analysis of FB poly(A)+ RNA showed it to be a part of the FTH transcript. Comparison of the 279-bp sequence with the GenBank and EMBL databases showed it to be 94.1, 62.5, and 58.9% similar to segments from human, mouse and rat FTH genomic sequences, respectively. However, in all these cases, only a part of this 279-bp sequence has been found in the nontranscribed region. We therefore conclude that in FB, the 279-bp sequence is a part of the mature FTH mRNA. Sequence analysis also suggests a differential poly(A) site selection in the production of FTH mRNA in FB and liver.
  • Recombinant DNA; λ Charon clones; poly(A)-site selection; extended untranslated region
Publication Date
April 30, 1993
Citation Information
Madhu S Dhar, V. Chauthaiwale and J. G. Joshi. "Sequence of a cDNA Encoding the Ferritin h-chain from an 11-week-old Human Fetal Brain" Gene Vol. 126 Iss. 2 (1993)
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