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The Human and Bovine 14-3-3η Protein mRNAs are Highly Conserved in both their Translated and Untranslated Regions
Biochimica et Biophysica Acta (1993)
  • K. D. Swanson, University of Tennessee, Knoxville
  • Madhu S Dhar, University of Tennessee, Knoxville
  • J. G. Joshi, University of Tennessee, Knoxville
Abstract
14-3-3 proteins form a highly conserved protein family whose members have been shown to activate tyrosine and tryptophan hydroxylases, inhibit protein kinase C and possess phospholipase A2 activity in vitro. We have isolated and analyzed a 14-3-3 protein cDNA clone (H14-3-3) from a human fetal brain cDNA library and found it to possess a high level of sequence identity with the bovine 14-3-3η protein cDNA in both the translated and untranslated regions, suggesting the presence of cis-regulatory elements in the untranslated regions of these mRNAs. The proteins encoded by these two cDNAs are 98.4% identical. Two different sized RNA species, approx. 1.9 and 3.5 kb in size that are expressed in a variety of tissues hybridize with this cDNA. However, only the 1.9 kb RNA is detected in the fetal brain. Northern blot analysis of poly(A)+ RNA isolated from eight different human tissues shows that 14-3-3 protein mRNAs are expressed in many tissues in the body. In agreement with previous reports, the highest abundance of RNA hybridizing with this cDNA is seen in the brain.
Keywords
  • Monoamine biosynthesis; Protein kinase C regulation; Phospoholipase A2; Signal transduction; mRNA untranslated region
Publication Date
October 19, 1993
Citation Information
K. D. Swanson, Madhu S Dhar and J. G. Joshi. "The Human and Bovine 14-3-3η Protein mRNAs are Highly Conserved in both their Translated and Untranslated Regions" Biochimica et Biophysica Acta Vol. 1216 Iss. 1 (1993)
Available at: http://works.bepress.com/madhu_dhar/31/