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Article
Impact of stress, fear and anxiety on the nociceptive responses of larval zebrafish
Sentience Collection
  • Javier Lopez-Luna, University of Liverpool
  • Qussay Al-Jubouri, University of Liverpool
  • Lynne U Sneddon, University of Liverpool
Document Type
Article
Publication Date
8-2-2017
Abstract

Both adult and larval zebrafish have been demonstrated to show behavioural responses to noxious stimulation but also to potentially stress- and fear or anxiety- eliciting situations. The pain or nociceptive response can be altered and modulated by these situations in adult fish through a mechanism called stress-induced analgesia. However, this phenomenon has not been described in larval fish yet. Therefore, this study explores the behavioural changes in larval zebrafish after noxious stimulation and exposure to challenges that can trigger a stress, fear or anxiety reaction. Five-day post fertilization zebrafish were exposed to either a stressor (air emersion), a predatory fear cue (alarm substance) or an anxiogenic (caffeine) alone or prior to immersion in acetic acid 0.1%. Pre- and post-stimulation behaviour (swimming velocity and time spent active) was recorded using a novel tracking software in 25 fish at once. Results show that larvae reduced both velocity and activity after exposure to the air emersion and alarm substance challenges and that these changes were attenuated using etomidate and diazepam, respectively. Exposure to acetic acid decreased velocity and activity as well, whereas air emersion and alarm substance inhibited these responses, showing no differences between pre- and post-stimulation. Therefore, we hypothesize that an antinociceptive mechanism, activated by stress and/or fear, occur in 5dpf zebrafish, which could have prevented the larvae to display the characteristic responses to pain.

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Copyright: © 2017 Lopez-Luna et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Citation Information
Lopez-Luna J, Al-Jubouri Q, Al-Nuaimy W, Sneddon LU (2017) Impact of stress, fear and anxiety on the nociceptive responses of larval zebrafish. PLoS ONE 12(8): e0181010. https://doi.org/10.1371/journal.pone.0181010