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Article
A membrane cytoskeleton from Dictyostelium discoideum. III. Plasma membrane fragments bind predominantly to the sides of actin filaments
Women’s Health Research Faculty Publications
  • C. M. Goodloe-Holland
  • Elizabeth J. Luna, University of Massachusetts Medical School
UMMS Affiliation
Department of Cell Biology
Date
7-1-1984
Document Type
Article
Subjects
Actins; Adenosine Triphosphate; Binding, Competitive; Cell Membrane; Cytoskeleton; Dictyostelium; Electrophoresis, Polyacrylamide Gel; Kinetics; Membrane Proteins; Microscopy, Electron; Myosin Subfragments; Myosins; Peptide Fragments
Abstract

The binding between sonicated Dictyostelium discoideum plasma membrane fragments and F-actin on Sephacryl S-1000 beads was found to be competitively inhibited by myosin subfragment-1. This inhibition is MgATP-sensitive, exhibits a Ki of approximately 5 X 10(-8) M, and is reciprocal, since membranes inhibit the binding of 125I-heavy meromyosin to F-actin on beads. These experiments demonstrate that membrane binding and S-1 binding to F-actin on beads are mutually exclusive and, therefore, that the membrane fragments bind predominantly to the sides, rather than to the ends, of the actin filaments. This conclusion is supported by electron micrographs that show many lateral associations between membrane fragments and bead-associated actin filaments. Such lateral associations could play an important role in the organization and lateral movement of membrane proteins by the cytomusculature.

Rights and Permissions
Citation: J Cell Biol. 1984 Jul;99(1 Pt 1):71-8. Link to article on publisher's website
Related Resources
Link to article in PubMed
PubMed ID
6610683
Citation Information
C. M. Goodloe-Holland and Elizabeth J. Luna. "A membrane cytoskeleton from Dictyostelium discoideum. III. Plasma membrane fragments bind predominantly to the sides of actin filaments" Vol. 99 Iss. 1 Pt 1 (1984) ISSN: 0021-9525 (Print)
Available at: http://works.bepress.com/lunae/28/