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Fluorescence Recovery after Photobleaching (FRAP) with a Focus on F-Actin
Current Protocols in Neuroscience
  • Lori R. Hardy, Philadelphia College of Osteopathic Medicine
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In this unit on fluorescence recovery after photobleaching (FRAP), an imaging approach to study protein-protein interactions in situ is described. The protocols presented use confocal microscopy to examine the mobility of a fluorescent protein by measuring the recovery of the protein in a bleached area. The data gained in FRAP studies is qualitative and yields insight into relative binding affinity, binding characteristics, and the effect of treatments or mutations on protein mobility.
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This article was published in Current Protocols in Neuroscience, Chapter 2, Unit 2.17, 2012.

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Copyright © 2012 by John Wiley & Sons, Inc.

Citation Information
Lori R. Hardy. Fluorescence Recovery after Photobleaching (FRAP) with a Focus on F-Actin. Current Protocols in Neuroscience Vol. Chapter 2 (2012) p. 2 - 2
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