Book
Fluorescence Recovery after Photobleaching (FRAP) with a Focus on F-Actin
Current Protocols in Neuroscience
Document Type
Book
Publication Date
1-1-2012
Disciplines
Abstract
In this unit on fluorescence recovery after photobleaching (FRAP), an imaging approach to study protein-protein interactions in situ is described. The protocols presented use confocal microscopy to examine the mobility of a fluorescent protein by measuring the recovery of the protein in a bleached area. The data gained in FRAP studies is qualitative and yields insight into relative binding affinity, binding characteristics, and the effect of treatments or mutations on protein mobility.
PubMed ID
23093350
Citation Information
Lori R. Hardy. Fluorescence Recovery after Photobleaching (FRAP) with a Focus on F-Actin. Current Protocols in Neuroscience Vol. Chapter 2 (2012) p. 2 - 2 Available at: http://works.bepress.com/lori_hardy/2/
This article was published in Current Protocols in Neuroscience, Chapter 2, Unit 2.17, 2012.
The published version is available at http://dx.doi.org/10.1002/0471142301.ns0217s61
Copyright © 2012 by John Wiley & Sons, Inc.