Fluorescence Recovery after Photobleaching (FRAP) with a Focus on F-ActinCurrent Protocols in Neuroscience
AbstractIn this unit on fluorescence recovery after photobleaching (FRAP), an imaging approach to study protein-protein interactions in situ is described. The protocols presented use confocal microscopy to examine the mobility of a fluorescent protein by measuring the recovery of the protein in a bleached area. The data gained in FRAP studies is qualitative and yields insight into relative binding affinity, binding characteristics, and the effect of treatments or mutations on protein mobility.
Citation InformationLori R. Hardy. Fluorescence Recovery after Photobleaching (FRAP) with a Focus on F-Actin. Current Protocols in Neuroscience Vol. Chapter 2 (2012) p. 2 - 2
Available at: http://works.bepress.com/lori_hardy/2/