Article
A Polyaniline-Based Optical Biosensing Platform Using an Entrapped Oxidoreductase Enzyme
Sensors and Actuators B: Chemical
Publication Date
9-21-2010
Keywords
- Glucose monitoring,
- Optical biosensor,
- Polyaniline,
- Glucose oxidase,
- Oxidoreductase,
- Gelation
Disciplines
Abstract
A novel optical biosensing platform utilizing the unique solubility and chemochromic properties of polyaniline is presented. A facile, ion-induced reprecipitation method leads to the entrapment of a chosen oxidoreductase enzyme, which, in the presence of its associated substrate, catalyzes a reversible redox change in the host polymer. This change is monitored via the UV–vis absorption and subsequently analyzed to fit a Michaelis–Menten model. Here, in vitro prototype devices demonstrate selective sensing of glucose, choline, and uric acid, and the potential to be adapted for use as part of real-time in vivo monitoring systems is discussed.
Additional Comments
IGERT grant #: 0221678; NS EC grant #: EEC-0425626
DOI
10.1016/j.snb.2010.06.054
Citation Information
Louis R. Nemzer and Arthur J. Epstein. "A Polyaniline-Based Optical Biosensing Platform Using an Entrapped Oxidoreductase Enzyme" Sensors and Actuators B: Chemical Vol. 150 Iss. 1 (2010) p. 376 - 383 ISSN: 0925-4005 Available at: http://works.bepress.com/lnemzer/28/
©2010 Elsevier B.V. All rights reserved.