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Porcine E. coli: Virulence-Associated Genes, Resistance Genes and Adhesion and Probiotic Activity Tested by a New Screening Method
PLoS ONE
  • Peter Schierack, Fakultät für Naturwissenschaften, Hochschule Lausitz (FH)
  • Stefan Rödiger, Fakultät für Naturwissenschaften, Hochschule Lausitz (FH)
  • Christoph Kuhl, Fakultät für Naturwissenschaften, Hochschule Lausitz (FH)
  • Rico Hiemann, Fakultät für Naturwissenschaften, Hochschule Lausitz (FH)
  • Dirk Roggenbuck, Fakultät für Naturwissenschaften, Hochschule Lausitz (FH)
  • Ganwu Li, Iowa State University
  • Jörg Weinreich, Fakultät für Naturwissenschaften, Hochschule Lausitz (FH)
  • Enrico Berger, Fakultät für Naturwissenschaften, Hochschule Lausitz (FH)
  • Lisa K. Nolan, Iowa State University
  • Bryon A. Nicholson, Iowa State University
  • Antje Römer, Federal Office of Consumer Protection and Food Safety
  • Ulrike Frömmel, Fakultät für Naturwissenschaften, Hochschule Lausitz (FH)
  • Lothar H. Wieler, Freie Universität Berlin
  • Christian Schröder, Fakultät für Naturwissenschaften, Hochschule Lausitz (FH)
Document Type
Article
Publication Date
4-26-2013
DOI
10.1371/journal.pone.0059242
Abstract

We established an automated screening method to characterize adhesion of Escherichia colito intestinal porcine epithelial cells (IPEC-J2) and their probiotic activity against infection by enteropathogenic E. coli (EPEC). 104 intestinal E. coli isolates from domestic pigs were tested by PCR for the occurrence of virulence-associated genes, genes coding for resistances to antimicrobial agents and metals, and for phylogenetic origin by PCR. Adhesion rates and probiotic activity were examined for correlation with the presence of these genes. Finally, data were compared with those from 93 E. coli isolates from wild boars.

Isolates from domestic pigs carried a broad variety of all tested genes and showed great diversity in gene patterns. Adhesions varied with a maximum of 18.3 or 24.2 mean bacteria adherence per epithelial cell after 2 or 6 hours respectively. Most isolates from domestic pigs and wild boars showed low adherence, with no correlation between adhesion/probiotic activity and E. coli genes or gene clusters. The gene sfa/foc, encoding for a subunit of F1C fimbriae did show a positive correlative association with adherence and probiotic activity; however E. coliisolates from wild boars with the sfa/foc gene showed less adhesion and probiotic activity thanE. coli with the sfa/foc gene isolated from domestic pigs after 6 hour incubation.

In conclusion, screening porcine E. coli for virulence associated genes genes, adhesion to intestinal epithelial cells, and probiotic activity revealed a single important adhesion factor, several probiotic candidates, and showed important differences between E. coli of domestic pigs and wild boars.

Comments

This article is from PLoS ONE 8, no. 4 (2013): e59242m, doi:10.1371/journal.pone.0059242.

Rights
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Copyright Owner
Schierack et al.
Language
en
Date Available
May 3, 2013
File Format
application/pdf
Citation Information
Peter Schierack, Stefan Rödiger, Christoph Kuhl, Rico Hiemann, et al.. "Porcine E. coli: Virulence-Associated Genes, Resistance Genes and Adhesion and Probiotic Activity Tested by a New Screening Method" PLoS ONE Vol. 8 Iss. 4 (2013) p. e59242 - e59242
Available at: http://works.bepress.com/lisa_nolan/9/