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Unpublished Paper
DNA Methylome in Spleen of Avian Pathogenic Escherichia coli-Challenged Broilers and Integration with mRNA Expression
Scientific Reports
  • Haiping Xu, South China Agricultural University
  • Xuenong Zhu, South China Agricultural University
  • Yongsheng Hu, South China Agricultural University
  • Zhenhui Li, South China Agricultural University
  • Xiquan Zhang, South China Agricultural University
  • Qinghua Nie, South China Agricultural University
  • Lisa K. Nolan, Iowa State University
  • Susan J. Lamont, Iowa State University
Document Type
Article
Publication Version
Published Version
Publication Date
3-1-2014
DOI
10.1038/srep04299
Abstract
Avian pathogenic Escherichia coli (APEC) are responsible for heavy economic losses in poultry industry. Here we investigate DNA methylome of spleen and identify functional DNA methylation changes related to host response to APEC among groups of non-challenged chickens (NC), challenged with mild (MD) and severe pathology (SV). DNA methylation was enriched in the gene bodies and repeats. Promoter and CGIs are hypomethylated. Integration analysis revealed 22, 87, and 9 genes exhibiting inversely changed DNA methylation and gene expression in NC vs. MD, NC vs. SV, and MD vs. SV, respectively.IL8, IL2RB, and IL1RAPL1 were included. Gene network analysis suggested that besides inflammatory response, other networks and pathways such as organismal injury and abnormalities, cell signaling and molecular transport, are probably related to host response to APEC infection. Moreover, methylation changes in cell cycle processes might contribute to the lesion phenotype differences between MD and SV.
Comments

This article is from Scientific Reports 4 (2014): 4299, doi:10.1038/srep04299. Posted with permission.

Rights
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Copyright Owner
Haiping Xu, et al
Language
en
File Format
application/pdf
Citation Information
Haiping Xu, Xuenong Zhu, Yongsheng Hu, Zhenhui Li, et al.. "DNA Methylome in Spleen of Avian Pathogenic Escherichia coli-Challenged Broilers and Integration with mRNA Expression" Scientific Reports Vol. 4 (2014) p. 1 - 10
Available at: http://works.bepress.com/lisa_nolan/86/