Comparison of a Quantitative Microtiter Method, a Quantitative Automated Method, and the Plate-Count Method for Determining Microbial Complement ResistanceAvian Diseases (1991)
AbstractA quantitative microtiter method for determining the degree of complement resistance or sensitivity of microorganisms is described. The microtiter method is compared with a quantitative automated system and the standard plate-count technique. Data were accumulated from 30 avian Escherichia coli isolates incubated at 35 C with either chicken plasma or heat-inactivated chicken plasma. Analysis of data generated by the automated system and plate-count techniques resulted in a classification of the microorganisms into three groups: those sensitive to the action of complement; those of intermediate sensitivity to the action of complement; and those resistant to the action of complement. Although the three methods studied did not agree absolutely, there were statistically significant correlations among them.
Publication DateDecember, 1991
Citation InformationMargie D. Lee, Richard E. Wooley, John Brown, Kathy R. Spears, et al.. "Comparison of a Quantitative Microtiter Method, a Quantitative Automated Method, and the Plate-Count Method for Determining Microbial Complement Resistance" Avian Diseases Vol. 35 Iss. 4 (1991)
Available at: http://works.bepress.com/lisa_nolan/74/