Transposon Mutagenesis Used to Study the Role of Complement Resistance in the Virulence of an Avian Escherichia coli IsolateAvian Diseases (1992)
AbstractThe role of complement resistance in the virulence of an avian Escherichia coli isolate was examined with transposon mutagenesis. A suicide plasmid containing a kanamycin-encoding mini-transposon was used to transform a virulent complement-resistant avian E. coli isolate. A less resistant mutant was identified that contained a transposon insertion in a plasmid and in the chromosome. This loss of complement resistance was associated with a drop in virulence in an embryo assay. No other phenotypic changes were detected in the mutant. These results suggest that complement resistance is associated with the virulence of this organism.
Publication DateJune, 1992
Citation InformationLisa K. Nolan, Richard E. Wooley and Richard K. Cooper. "Transposon Mutagenesis Used to Study the Role of Complement Resistance in the Virulence of an Avian Escherichia coli Isolate" Avian Diseases Vol. 36 Iss. 2 (1992)
Available at: http://works.bepress.com/lisa_nolan/73/