Effect of Normal Intestinal Flora of Chickens on Colonization by Virulent Colicin V-Producing, Avirulent, and Mutant Colicin V-Producing Avian Escherichia coliAvian Diseases (1994)
AbstractColonization of the intestinal tracts of newly hatched chicks with Escherichia coli was attempted by swabbing test organisms onto the air-shell of 19-day-old embryos. Test organisms consisted of two virulent E. coli isolates, one avirulent isolate, and one laboratory-derived mutant of the avirulent isolate carrying a recombinant plasmid coding for Colicin V production. Chicks were cultured weekly for 3 weeks for total E. coliand for the test organisms using selective media. Control chicks were sampled on weeks 1 and 5, and the normal E. coli intestinal microflora were examined for the production of colicins. The two virulent E. coli isolates maintained colonization of the chicks for the 3-week test period, with titers decreasing from 10' to 10' colony-forming units (CFU)/g of intestine. The avirulent isolate and laboratory mutant did not consistently colonize the intestinal tracts. The majority of intestinal samples taken from the control chicks at 1 and 5 weeks had colicin-producing E. coli that were inhibitory to the test organisms.
Publication DateMarch, 1994
Citation InformationRichard E. Wooley, John Brown, Penelope S. Gibbs, Lisa K. Nolan, et al.. "Effect of Normal Intestinal Flora of Chickens on Colonization by Virulent Colicin V-Producing, Avirulent, and Mutant Colicin V-Producing Avian Escherichia coli" Avian Diseases Vol. 38 Iss. 1 (1994)
Available at: http://works.bepress.com/lisa_nolan/66/