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Phenotypic Expression of Recombinant Plasmids pKT107 and pHK11 in an Avirulent Avian Escherichia coli
Avian Diseases (1994)
  • Richard E. Wooley, University of Georgia
  • Lisa K. Nolan, North Dakota State University
  • John Brown, University of Georgia
  • Penelope S. Gibbs, University of Georgia
  • Denise I. Bounous, University of Georgia
An avirulent wild-type avian Escherichia coli strain (Av) was electrotransformed with plasmids coding for complement resistance (pKT107) and Colicin V (ColV) production (pHK11) in order to study the effects of complement resistance and ColV production on virulence. Transformants were also compared with the wild type for embryo lethality, uptake by macrophages, motility, growth rate, plasmid content, and hemolysis. Growth rates and complement resistance patterns of strain Av and transformant Av+pHK1 1 were similar, but Av+pHK11 caused a significantly greater number of deaths in embryos and acquired motility. Transformant Av+pKT107 had a lower rate of phagocytosis, a slower growth rate, and a greater sensitivity to complement, and it changed from being non-hemolytic to expressing a-hemolytic action. The 35-kb plasmid present in the wild type was not present in the transformants. Although some of the results demonstrate the difficulties encountered in using wild-type organisms as recipients in virulence studies, the results with Av+pHK11 indicate that ColV production plus the acquisition of motility contributes to the virulence of avian E. coli.
Publication Date
March, 1994
Publisher Statement
Copyright 1994 American Association of Avian Pathologists. Posted with permission.
Citation Information
Richard E. Wooley, Lisa K. Nolan, John Brown, Penelope S. Gibbs, et al.. "Phenotypic Expression of Recombinant Plasmids pKT107 and pHK11 in an Avirulent Avian Escherichia coli" Avian Diseases Vol. 38 Iss. 1 (1994)
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