Invasion of Caco-2 Cells by Salmonella typhimurium (Copenhagen) Isolates from Healthy and Sick ChickensAvian Diseases (1995)
AbstractIn a previous study, Salmonella isolates of sick birds were distinguished from those of apparently healthy birds by their high degree of invasion of tissue culture cells. In this study, a single pair of Salmonella isolates was examined to determine the source of this observed difference in invasion. When isolates were allowed to invade Caco-2 cells for 8 hours, the isolate from the sick bird (S) appeared to invade in greater numbers than did the isolate from the healthy bird (H). However, when invasion was distinguished from intracellular growth/survival, it was found that H invaded in greater numbers than S, but once inside the cell, H declined in number, and S increased. Inhibition of RNA, protein, and DNA syntheses lessened the degree to which both invaded. The presence of mannose inhibited invasion by S but did not appear to inhibit invasion by H. Trypsin treatment of monolayers affected invasion of S and H, whereas neuraminidase treatment did not. There was no significant difference noted between S and H in ability to adhere to fixed monolayers. Therefore, the two isolates tested differ in their mechanisms of entry into Caco-2 cells, the efficiency with which they invade, and their ability to survive withinCaco-2 cells.
Publication DateDecember, 1995
Citation InformationTheodore J. Kottom, Lisa K. Nolan and John Brown. "Invasion of Caco-2 Cells by Salmonella typhimurium (Copenhagen) Isolates from Healthy and Sick Chickens" Avian Diseases Vol. 39 Iss. 4 (1995)
Available at: http://works.bepress.com/lisa_nolan/64/