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Sequence Analysis and Characterization of a Transferable Hybrid Plasmid Encoding Multidrug Resistance and Enabling Zoonotic Potential for Extraintestinal Escherichia coli
Infection and Immunity
  • Timothy J. Johnson, Iowa State University
  • Dianna Jordan, Iowa State University
  • Subhashinie Kariyawasam, Iowa State University
  • Adam L. Stell, University of Minnesota
  • Nathan P. Bell, University of Minnesota
  • Yvonne Wannemuehler, Iowa State University
  • Claudia Fernández Alarcón, University of Minnesota
  • Ganwu Li, Iowa State University
  • Kelly A. Tivendale, Iowa State University
  • Catherine M. Logue, North Dakota State University
  • Lisa K. Nolan, Iowa State University
Document Type
Article
Publication Date
2-1-2010
DOI
10.1128/​IAI.01174-09
Abstract
ColV plasmids of extraintestinal pathogenic Escherichia coli (ExPEC) encode a variety of fitness and virulence factors and have long been associated with septicemia and avian colibacillosis. These plasmids are found significantly more often in ExPEC, including ExPEC associated with human neonatal meningitis and avian colibacillosis, than in commensal E. coli. Here we describe pAPEC-O103-ColBM, a hybrid RepFIIA/FIB plasmid harboring components of the ColV pathogenicity island and a multidrug resistance (MDR)-encoding island. This plasmid is mobilizable and confers the ability to cause septicemia in chickens, the ability to cause bacteremia resulting in meningitis in the rat model of human disease, and the ability to resist the killing effects of multiple antimicrobial agents and human serum. The results of a sequence analysis of this and other ColV plasmids supported previous findings which indicated that these plasmid types arose from a RepFIIA/FIB plasmid backbone on multiple occasions. Comparisons of pAPEC-O103-ColBM with other sequenced ColV and ColBM plasmids indicated that there is a core repertoire of virulence genes that might contribute to the ability of some ExPEC strains to cause high-level bacteremia and meningitis in a rat model. Examination of a neonatal meningitis E. coli(NMEC) population revealed that approximately 58% of the isolates examined harbored ColV-type plasmids and that 26% of these plasmids had genetic contents similar to that of pAPEC-O103-ColBM. The linkage of the ability to confer MDR and the ability contribute to multiple forms of human and animal disease on a single plasmid presents further challenges for preventing and treating ExPEC infections.
Comments

This article is from Infection and Immunity 78, no. 5 (May 2010): 1931–1942, doi:10.1128/IAI.01174-09.

Copyright Owner
American Society for Microbiology
Language
en
File Format
application/pdf
Citation Information
Timothy J. Johnson, Dianna Jordan, Subhashinie Kariyawasam, Adam L. Stell, et al.. "Sequence Analysis and Characterization of a Transferable Hybrid Plasmid Encoding Multidrug Resistance and Enabling Zoonotic Potential for Extraintestinal Escherichia coli" Infection and Immunity Vol. 78 Iss. 5 (2010) p. 1931 - 1942
Available at: http://works.bepress.com/lisa_nolan/6/