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Article
Pap Mutant of Avian Pathogenic Escherichia coli O1, an O1:K1:H7 Strain, Is Attenuated In Vivo
Avian Diseases
  • Subhashinie Kariyawasam, Iowa State University
  • Lisa K. Nolan, Iowa State University
Document Type
Article
Publication Date
6-1-2009
DOI
10.1637/8519-111708-Reg.1
Abstract
P or Pap fimbriae of avian pathogenic Escherichia coli (APEC) have previously been shown to be important in the pathogenesis of colibacillosis. However, no study has been done to ascertain the effects of deletion of the genes responsible for P fimbrial biogenesis on APEC'sin vitro and/or in vivo characteristics. In the present study, all 11 genes of the pap gene cluster were deleted from APEC O1, the recently sequenced APEC strain, and the wild-type strain was compared to the mutant (ECPAP1) for changes in certain phenotypic characteristics and virulence for chickens. Both APEC O1 and ECPAP1 demonstrated mannose-sensitive agglutination of guinea pig and chicken erythrocytes, but only APEC O1 demonstrated mannose-resistant hemagglutination and P-receptor binding properties. The in vivo experiments revealed that ECPAP1 was markedly attenuated as compared to its wild-type strain APEC O1. These findings suggest that P fimbriae are involved in the virulence of APEC O1.
Comments

This article is from Avian Diseases 53, no. 2 (2009): 255–260, doi:10.1637/8519-111708-Reg.1.

Copyright Owner
American Association of Avian Pathologists
Language
en
File Format
application/pdf
Citation Information
Subhashinie Kariyawasam and Lisa K. Nolan. "Pap Mutant of Avian Pathogenic Escherichia coli O1, an O1:K1:H7 Strain, Is Attenuated In Vivo" Avian Diseases Vol. 53 Iss. 2 (2009) p. 255 - 260
Available at: http://works.bepress.com/lisa_nolan/42/