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Associations Between Multidrug Resistance, Plasmid Content, and Virulence Potential Among Extraintestinal Pathogenic and Commensal Escherichia coli from Humans and Poultry
Foodborne Pathogens and Disease
  • Timothy J. Johnson, Iowa State University
  • Catherine M. Logue, North Dakota State University
  • James R. Johnson, University of Minnesota
  • Michael A. Kuskowski, University of Minnesota
  • Julie S. Sherwood, North Dakota State University
  • H. John Barnes, North Carolina State University
  • Chitrita DebRoy, Pennsylvania State University
  • Yvonne Wannemuehler, Iowa State University
  • Mana Obata-Yasuoka, University of Tsukuba
  • Lodewijk Spanjaard, Academic Medical Center
  • Lisa K. Nolan, Iowa State University
Document Type
Article
Publication Date
1-4-2012
DOI
10.1089/fpd.2011.0961
Abstract
The emergence of plasmid-mediated multidrug resistance (MDR) among enteric bacteria presents a serious challenge to the treatment of bacterial infections in humans and animals. Recent studies suggest that avianEscherichia coli commonly possess the ability to resist multiple antimicrobial agents, and might serve as reservoirs of MDR for human extraintestinal pathogenic Escherichia coli (ExPEC) and commensal E. coli populations. We determined antimicrobial susceptibility profiles for 2202 human and avian E. coli isolates, then sought for associations among resistance profile, plasmid content, virulence factor profile, and phylogenetic group. Avian-source isolates harbored greater proportions of MDR than their human counterparts, and avian ExPEC had higher proportions of MDR than did avian commensal E. coli. MDR was significantly associated with possession of the IncA/C, IncP1-α, IncF, and IncI1 plasmid types. Overall, inferred virulence potential did not correlate with drug susceptibility phenotype. However, certain virulence genes were positively associated with MDR, including ireA,ibeA, fyuA, cvaC, iss, iutA, iha, and afa. According to the total dataset, isolates segregated significantly according to host species and clinical status, thus suggesting that avian and human ExPEC and commensal E. coli represent four distinct populations with limited overlap. These findings suggest that in extraintestinal E. coli, MDR is most commonly associated with plasmids, and that these plasmids are frequently found among avian-source E. coli from poultry production systems.
Comments

This is a copy of an article published in Foodborne Pathogens and Disease © 2012 Mary Ann Liebert, Inc; Foodborne Pathogens and Disease is available online at http://online.liebertpub.com.

Copyright Owner
Mary Ann Liebert, Inc.
Language
en
Date Available
May 6, 2013
File Format
application/pdf
Citation Information
Timothy J. Johnson, Catherine M. Logue, James R. Johnson, Michael A. Kuskowski, et al.. "Associations Between Multidrug Resistance, Plasmid Content, and Virulence Potential Among Extraintestinal Pathogenic and Commensal Escherichia coli from Humans and Poultry" Foodborne Pathogens and Disease Vol. 9 Iss. 1 (2012) p. 37 - 46
Available at: http://works.bepress.com/lisa_nolan/35/