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Article
DNA Sequence of a ColV Plasmid and Prevalence of Selected Plasmid-Encoded Virulence Genes among Avian Escherichia coli Strains
Journal of Bacteriology
  • Timothy J. Johnson, Iowa State University
  • Kylie E. Siek, Iowa State University
  • Sara J. Johnson, Iowa State University
  • Lisa K. Nolan, Iowa State University
Document Type
Article
Publication Date
1-1-2006
DOI
10.1128/​JB.188.2.745-758.2006
Abstract
olV plasmids have long been associated with the virulence of Escherichia coli, despite the fact that their namesake trait, ColV production, does not appear to contribute to virulence. Such plasmids or their associated sequences appear to be quite common among avian pathogenic E. coli (APEC) and are strongly linked to the virulence of these organisms. In the present study, a 180-kb ColV plasmid was sequenced and analyzed. This plasmid, pAPEC-O2-ColV, possesses a 93-kb region containing several putative virulence traits, including iss, tsh, and four putative iron acquisition and transport systems. The iron acquisition and transport systems include those encoding aerobactin and salmochelin, the sitABC iron transport system, and a putative iron transport system novel to APEC,eit. In order to determine the prevalence of the virulence-associated genes within this region among avian E. coli strains, 595 APEC and 199 avian commensal E. coli isolates were examined for genes of this region using PCR. Results indicate that genes contained within a portion of this putative virulence region are highly conserved among APEC and that the genes of this region occur significantly more often in APEC than in avian commensal E. coli. The region of pAPEC-O2-ColV containing genes that are highly prevalent among APEC appears to be a distinguishing trait of APEC strains.
Comments

This article is from Journal of Bacteriology 188, no. 2 (January 2006): 745–758, doi:10.1128/JB.188.2.745-758.2006.

Copyright Owner
American Society for Microbiology
Language
en
Date Available
May 6, 2013
File Format
application/pdf
Citation Information
Timothy J. Johnson, Kylie E. Siek, Sara J. Johnson and Lisa K. Nolan. "DNA Sequence of a ColV Plasmid and Prevalence of Selected Plasmid-Encoded Virulence Genes among Avian Escherichia coli Strains" Journal of Bacteriology Vol. 188 Iss. 2 (2006) p. 745 - 758
Available at: http://works.bepress.com/lisa_nolan/31/