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Transcriptome Analysis of Avian Pathogenic Escherichia coli O1 in Chicken Serum Reveals Adaptive Responses to Systemic Infection
Infection and Immunity
  • Ganwu Li, Iowa State University
  • Kelly A. Tivendale, Iowa State University
  • Peng Liu, Iowa State University
  • Yaping Feng, Iowa State University
  • Yvonne Wannemuehler, Iowa State University
  • Wentong Cai, Iowa State University
  • Paul M. Mangiamele, Iowa State University
  • Timothy J. Johnson, University of Minnesota
  • Chrystala Constantinidou, University of Birmingham
  • Charles W. Penn, University of Birmingham
  • Lisa K. Nolan, Iowa State University
Document Type
Article
Publication Date
5-1-2011
DOI
10.1128/​IAI.01230-10
Abstract

Infections of avian pathogenic Escherichia coli (APEC) result in annual multimillion-dollar losses to the poultry industry. Despite this, little is known about the mechanisms by which APEC survives and grows in the bloodstream. Thus, the aim of this study was to identify molecular mechanisms enabling APEC to survive and grow in this critical host environment. To do so, we compared the transcriptome of APEC O1 during growth in Luria-Bertani broth and chicken serum. Several categories of genes, predicted to contribute to adaptation and growth in the avian host, were identified. These included several known virulence genes and genes involved in adaptive metabolism, protein transport, biosynthesis pathways, stress resistance, and virulence regulation. Several genes with unknown function, which were localized to pathogenicity islands or APEC O1's large virulence plasmid, pAPEC-O1-ColBM, were also identified, suggesting that they too contribute to survival in serum. The significantly upregulated genesdnaK, dnaJ, phoP, and ybtA were subsequently subjected to mutational analysis to confirm their role in conferring a competitive advantage during infection. This genome-wide analysis provides novel insight into processes that are important to the pathogenesis of APEC O1.

Comments

This article is from Infection and Immunity 79, no. 5 (May 2011): 1951–1960, doi:10.1128/IAI.01230-10.

Copyright Owner
American Society for Microbiology
Language
en
Date Available
May 3, 2013
File Format
application/pdf
Citation Information
Ganwu Li, Kelly A. Tivendale, Peng Liu, Yaping Feng, et al.. "Transcriptome Analysis of Avian Pathogenic Escherichia coli O1 in Chicken Serum Reveals Adaptive Responses to Systemic Infection" Infection and Immunity Vol. 79 Iss. 5 (2011) p. 1951 - 1960
Available at: http://works.bepress.com/lisa_nolan/3/