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The Genome Sequence of Avian Pathogenic Escherichia coli Strain O1:K1:H7 Shares Strong Similarities with Human Extraintestinal Pathogenic E. coli Genomes
Journal of Bacteriology
  • Timothy J. Johnson, Iowa State University
  • Subhashinie Kariyawasam, Iowa State University
  • Yvonne Wannemuehler, Iowa State University
  • Paul M. Mangiamele, Iowa State University
  • Sara J. Johnson, Iowa State University
  • Curt Doetkott, North Dakota State University
  • Jerod A. Skyberg, Iowa State University
  • Aaron M. Lynne, Iowa State University
  • James R. Johnson, University of Minnesota
  • Lisa K. Nolan, Iowa State University
Document Type
Article
Publication Date
4-1-2007
DOI
10.1128/​JB.01726-06
Abstract

Escherichia coli strains that cause disease outside the intestine are known as extraintestinal pathogenic E. coli (ExPEC) and include human uropathogenic E. coli(UPEC) and avian pathogenic E. coli (APEC). Regardless of host of origin, ExPEC strains share many traits. It has been suggested that these commonalities may enable APEC to cause disease in humans. Here, we begin to test the hypothesis that certain APEC strains possess potential to cause human urinary tract infection through virulence genotyping of 1,000 APEC and UPEC strains, generation of the first complete genomic sequence of an APEC (APEC O1:K1:H7) strain, and comparison of this genome to all available human ExPEC genomic sequences. The genomes of APEC O1 and three human UPEC strains were found to be remarkably similar, with only 4.5% of APEC O1's genome not found in other sequenced ExPEC genomes. Also, use of multilocus sequence typing showed that some of the sequenced human ExPEC strains were more like APEC O1 than other human ExPEC strains. This work provides evidence that at least some human and avian ExPEC strains are highly similar to one another, and it supports the possibility that a food-borne link between some APEC and UPEC strains exists. Future studies are necessary to assess the ability of APEC to overcome the hurdles necessary for such a food-borne transmission, and epidemiological studies are required to confirm that such a phenomenon actually occurs.

Comments

This article is from Journal of Bacteriology 189, no. 8 (April 2007): 3228–3236, doi:10.1128/JB.01726-06.

Copyright Owner
American Society for Microbiology
Language
en
Date Available
May 6, 2013
File Format
application/pdf
Citation Information
Timothy J. Johnson, Subhashinie Kariyawasam, Yvonne Wannemuehler, Paul M. Mangiamele, et al.. "The Genome Sequence of Avian Pathogenic Escherichia coli Strain O1:K1:H7 Shares Strong Similarities with Human Extraintestinal Pathogenic E. coli Genomes" Journal of Bacteriology Vol. 189 Iss. 8 (2007) p. 3228 - 3236
Available at: http://works.bepress.com/lisa_nolan/29/