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Article
Evolution of the iss Gene in Escherichia coli
Applied and Environmental Microbiology
  • Timothy J. Johnson, University of Minnesota
  • Yvonne Wannemuehler, Iowa State University
  • Lisa K. Nolan, Iowa State University
Document Type
Article
Publication Date
4-1-2008
DOI
10.1128/​AEM.02634-07
Abstract

The increased serum survival gene iss has long been recognized for its role in extraintestinal pathogenic Escherichia coli (ExPEC) virulence. iss has been identified as a distinguishing trait of avian ExPEC but not of human ExPEC. This gene has been localized to large virulence plasmids and shares strong similarities with the bor gene from bacteriophage λ. Here, we demonstrate that three alleles of iss occur among E. coli isolates that appear to have evolved from a common λbor precursor. In addition to the occurrence of iss on the ColV/BM virulence plasmids, at least two iss alleles occur within the E. coli chromosome. One of these alleles (designated type 3) was found to occur in the genomes of all currently sequenced ExPEC strains on a similar prophage element that also harbors the Sit iron and manganese transport system. When the prevalence of the three iss types was examined among 487 E. coli isolates, the iss type 3 gene was found to occur at a high frequency among ExPEC isolates, irrespective of the host source. The plasmid-borne iss allele (designated type 1) was highly prevalent among avian pathogenic E. coli and neonatal meningitis-associated E. coliisolates but not among uropathogenic E. coli isolates. This study demonstrates the evolution of iss in E. coli and provides an additional tool for discriminating among E. coli pathotypes through the differentiation of the three iss allele types and bor.

Comments

This article is from Applied and Environmental Microbiology 74, no. 8 (April 2008): 2360–2369, doi:10.1128/AEM.02634-07.

Copyright Owner
American Society for Microbiology
Language
en
Date Available
May 6, 2013
File Format
application/pdf
Citation Information
Timothy J. Johnson, Yvonne Wannemuehler and Lisa K. Nolan. "Evolution of the iss Gene in Escherichia coli" Applied and Environmental Microbiology Vol. 74 Iss. 8 (2008) p. 2360 - 2369
Available at: http://works.bepress.com/lisa_nolan/26/