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Article
ultilocus Sequence Typing Lacks the Discriminatory Ability of Pulsed-Field Gel Electrophoresis for Typing Salmonella enterica Serovar Typhimurium
Journal of Clinical Microbiology
  • Mohamed K. Fakhr, North Dakota State University
  • Lisa K. Nolan, Iowa State University
  • Catherine M. Logue, North Dakota State University
Document Type
Article
Publication Date
5-1-2005
DOI
10.1128/​JCM.43.5.2215-2219.2005
Abstract

Nontyphoidal salmonellae are among the leading causes of food-borne disease in the United States. Because of the importance of Salmonella enterica in food-borne disease, numerous typing methodologies have been developed. Among the several molecular typing methods, pulsed-field gel electrophoresis (PFGE) is currently considered the “gold standard” technique in typing Salmonella. The aim of this study was to compare the discriminatory power of PFGE to multilocus sequence typing (MLST) in typing Salmonella enterica serovar Typhimurium clinical isolates. A total of 85 Salmonella Typhimurium clinical isolates from cattle were used in this study. PFGE using XbaI was performed on the 85 isolates by the Centers for Disease Control and Prevention method, and data were analyzed using the BioNumerics software package. Fifty PFGE profiles were observed among the isolates, and these grouped into three major clusters. For the MLST analysis, the manB, pduF, glnA, and spaM genes were amplified by PCR from the same 85 isolates. DNA sequencing of these four genes, manB, pduF, glnA, andspaM, showed no genetic diversity among the isolates tested, with a 100% identity in nucleotide sequence. Moreover, the DNA sequences of the aforementioned genes showed 100% identity to the sequence reported in GenBank for the S. enterica serovar Typhimurium LT2 strain. Therefore, MLST, using these genes, lacks the discriminatory power of PFGE for typing Salmonella entericaserovar Typhimurium.

Comments

This article is from Journal of Clinical Microbiology 43, no. 5 (May 2005): 2215–2219, doi:10.1128/JCM.43.5.2215-2219.2005.

Copyright Owner
American Society for Microbiology
Language
en
Date Available
May 6, 2013
File Format
application/pdf
Citation Information
Mohamed K. Fakhr, Lisa K. Nolan and Catherine M. Logue. "ultilocus Sequence Typing Lacks the Discriminatory Ability of Pulsed-Field Gel Electrophoresis for Typing Salmonella enterica Serovar Typhimurium" Journal of Clinical Microbiology Vol. 43 Iss. 5 (2005) p. 2215 - 2219
Available at: http://works.bepress.com/lisa_nolan/20/