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Genotypic and Phenotypic Traits That Distinguish Neonatal Meningitis-Associated Escherichia coli from Fecal E. coli Isolates of Healthy Human Hosts
Applied and Environmental Microbiology
  • Catherine M. Logue, Iowa State University
  • Curt Doetkott, North Dakota State University
  • Paul M. Mangiamele, Iowa State University
  • Yvonne Wannemuehler, Iowa State University
  • Timothy J. Johnson, University of Minnesota
  • Kelly A. Tivendale, Iowa State University
  • Ganwu Li, Iowa State University
  • Julie S. Sherwood, North Dakota State University
  • Lisa K. Nolan, Iowa State University
Document Type
Article
Publication Date
8-1-2012
DOI
10.1128/​AEM.07869-11
Abstract

Neonatal meningitis Escherichia coli (NMEC) is one of the top causes of neonatal meningitis worldwide. Here, 85 NMEC and 204 fecal E. coli isolates from healthy humans (HFEC) were compared for possession of traits related to virulence, antimicrobial resistance, and plasmid content. This comparison was done to identify traits that typify NMEC and distinguish it from commensal strains to refine the definition of the NMEC subpathotype, identify traits that might contribute to NMEC pathogenesis, and facilitate choices of NMEC strains for future study. A large number of E. coli strains from both groups were untypeable, with the most common serogroups occurring among NMEC being O18, followed by O83, O7, O12, and O1. NMEC strains were more likely than HFEC strains to be assigned to the B2 phylogenetic group. Few NMEC or HFEC strains were resistant to antimicrobials. Genes that best discriminated between NMEC and HFEC strains and that were present in more than 50% of NMEC isolates were mainly from extraintestinal pathogenic E. coli genomic and plasmid pathogenicity islands. Several of these defining traits had not previously been associated with NMEC pathogenesis, are of unknown function, and are plasmid located. Several genes that had been previously associated with NMEC virulence did not dominate among the NMEC isolates. These data suggest that there is much about NMEC virulence that is unknown and that there are pitfalls to studying single NMEC isolates to represent the entire subpathotype.

Comments

This article is from Applied and Environmental Microbiology 78, no. 16 (August 2012): 5824–5830, doi:10.1128/AEM.07869-11.

Copyright Owner
American Society for Microbiology
Language
en
Date Available
May 6, 2013
File Format
application/pdf
Citation Information
Catherine M. Logue, Curt Doetkott, Paul M. Mangiamele, Yvonne Wannemuehler, et al.. "Genotypic and Phenotypic Traits That Distinguish Neonatal Meningitis-Associated Escherichia coli from Fecal E. coli Isolates of Healthy Human Hosts" Applied and Environmental Microbiology Vol. 78 Iss. 16 (2012) p. 5824 - 5830
Available at: http://works.bepress.com/lisa_nolan/19/