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Clonal Spread of Methicillin-resistant Staphylococcus pseudintermedius in Europe and North America: an International Multicentre Study
Journal of Antimicrobial Chemotherapy (2010)
  • Vincent Perreten
  • Kristina Kadlec
  • Stefan Schwarz
  • Ulrika Gronlund Andersson
  • Maria Finn
  • Christina Greko
  • Arshnee Moodley, University of Copenhagen
  • Stephen A. Kania, University of Tennessee - Knoxville
  • Linda Frank, University of Tennessee, Knoxville
  • David A. Bemis, University of Tennessee - Knoxville
  • Alessia Franco
  • Manuala Lurescia
  • Antonio Battisti
  • Birgitta Duim, Utrecht University
  • Jaap A. Wagenaar, Utrecht University
  • Engeline van Duijkeren, Utrecht University
  • J. Scott Weese, University of Guelph
  • J. Ross Fitzgerald, University of Edinburgh
  • Alexandra Rossano
  • Luca Guardabassi, University of Copenhagen
Abstract
Objectives The aim of this study was to determine the phenotypic and genotypic resistance profiles of methicillin-resistant Staphylococcus pseudintermedius (MRSP) and to examine the clonal distribution in Europe and North America. Methods A total of 103 MRSP isolates from dogs isolated from several countries in Europe, the USA and Canada were characterized. Isolates were identified by PCR–restriction fragment length polymorphism (RFLP), antimicrobial susceptibility was determined by broth dilution or gradient diffusion, and antimicrobial resistance genes were detected using a microarray. Genetic diversity was assessed by multilocus sequence typing (MLST), PFGE and spa typing. Staphylococcal cassette chromosome mec (SCCmec) elements were characterized by multiplex PCR. Results Thirteen different sequence types (STs), 18 PFGE types and 8 spa types were detected. The hybrid SCCmec element II–III described in a MRSP isolate was present in 75 (72.8%) isolates. The remaining isolates either had SCCmec type III (n = 2), IV (n = 6), V (n = 14) or VII-241 (n = 4) or were non-typeable (n = 2). The most common genotypes were ST71(MLST)-J(PFGE)-t02(spa)-II–III(SCCmec) (56.3%) and ST68-C-t06-V (12.6%). In addition to mecA-mediated β-lactam resistance, isolates showed resistance to trimethoprim [dfr(G)] (90.3%), gentamicin/kanamycin [aac(6′)-Ie–aph(2′)-Ia] (88.3%), kanamycin [aph(3′)-III] (90.3%), streptomycin [ant(6′)-Ia] (90.3%), streptothricin (sat4) (90.3%), macrolides and/or lincosamides [erm(B), lnu(A)] (89.3%), fluoroquinolones (87.4%), tetracycline [tet(M) and/or tet(K)] (69.9%), chloramphenicol (catpC221) (57.3%) and rifampicin (1.9%). Conclusions Two major clonal MRSP lineages have disseminated in Europe (ST71-J-t02-II–III) and North America (ST68-C-t06-V). Regardless of their geographical or clonal origin, the isolates displayed resistance to the major classes of antibiotics used in veterinary medicine and thus infections caused by MRSP isolates represent a serious therapeutic challenge.
Keywords
  • antimicrobial resistance,
  • genotyping,
  • MLST,
  • PFGE,
  • spa,
  • dog
Publication Date
June, 2010
Citation Information
Vincent Perreten, Kristina Kadlec, Stefan Schwarz, Ulrika Gronlund Andersson, et al.. "Clonal Spread of Methicillin-resistant Staphylococcus pseudintermedius in Europe and North America: an International Multicentre Study" Journal of Antimicrobial Chemotherapy Vol. 65 Iss. 6 (2010)
Available at: http://works.bepress.com/linda_frank/16/