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Article
Calcium signaling in endocardial and epicardial ventricular myocytes from streptozotocin-induced diabetic rats
Journal of Diabetes Investigation
  • Lina T. Al Kury, Zayed University
  • Vadym Sydorenko, Bogomoletz Institute of Physiology
  • Manal M.A. Smail, College of Medicine and Health Sciences United Arab Emirates University
  • Muhammad A. Qureshi, College of Medicine and Health Sciences United Arab Emirates University
  • Anatoly Shmygol, College of Medicine and Health Sciences United Arab Emirates University
  • Dimitrios Papandreou, Zayed University
  • Jaipaul Singh, University of Central Lancashire
  • Frank Christopher Howarth, College of Medicine and Health Sciences United Arab Emirates University
ORCID Identifiers

0000-0002-8338-7655

Document Type
Article
Publication Date
1-1-2020
Abstract

© 2020 The Authors. Journal of Diabetes Investigation published by Asian Association for the Study of Diabetes (AASD) and John Wiley & Sons Australia, Ltd Aims/Introduction: Abnormalities in Ca2+ signaling have a key role in hemodynamic dysfunction in diabetic heart. The purpose of this study was to explore the effects of streptozotocin (STZ)-induced diabetes on Ca2+ signaling in epicardial (EPI) and endocardial (ENDO) cells of the left ventricle after 5–6 months of STZ injection. Materials and Methods: Whole-cell patch clamp was used to measure the L-type Ca2+ channel (LTCC) and Na+/Ca2+ exchanger currents. Fluorescence photometry techniques were used to measure intracellular free Ca2+ concentration. Results: Although the LTCC current was not significantly altered, the amplitude of Ca2+ transients increased significantly in EPI-STZ and ENDO-STZ compared with controls. Time to peak LTCC current, time to peak Ca2+ transient, time to half decay of LTCC current and time to half decay of Ca2+ transients were not significantly changed in EPI-STZ and ENDO-STZ myocytes compared with controls. The Na+/Ca2+ exchanger current was significantly smaller in EPI-STZ and in ENDO-STZ compared with controls. Conclusions: STZ-induced diabetes resulted in an increase in amplitude of Ca2+ transients in EPI and ENDO myocytes that was independent of the LTCC current. Such an effect can be attributed, at least in part, to the dysfunction of the Na+/Ca2+ exchanger. Additional studies are warranted to improve our understanding of the regional impact of diabetes on Ca2+ signaling, which will facilitate the discovery of new targeted treatments for diabetic cardiomyopathy.

Publisher
Wiley
Keywords
  • Ca transients 2+,
  • Na /Ca exchanger + 2+,
  • Streptozotocin-induced diabetes
Scopus ID

85096921409

Creative Commons License
Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International
Indexed in Scopus
Yes
Open Access
Yes
Open Access Type
Gold: This publication is openly available in an open access journal/series
Citation Information
Lina T. Al Kury, Vadym Sydorenko, Manal M.A. Smail, Muhammad A. Qureshi, et al.. "Calcium signaling in endocardial and epicardial ventricular myocytes from streptozotocin-induced diabetic rats" Journal of Diabetes Investigation (2020) ISSN: <p><a href="https://v2.sherpa.ac.uk/id/publication/issn/2040-1116" target="_blank">2040-1116</a></p>
Available at: http://works.bepress.com/lina-alkury/26/