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Article
Molecular Basis for Strain Variation in the S. Cerevisiae adhesion Flo11p
MSphere
  • Li Li, Ph.D., Molloy College
  • Subit Barua
  • Peter N Lipke
  • Anne M Dranginis
Document Type
Peer-Reviewed Article
Publication Date
8-1-2016
Version
Publisher's PDF
Publisher's Statement
ASM grants authors the right to post their accepted manuscripts in publicly accessible electronic repositories maintained by funding agencies, as well as appropriate institutional or subject-based open repositories established by a government or non-commercial entity.
Abstract

FLO11 encodes a yeast cell wall flocculin that mediates a variety of adhesive phenotypes in Saccharomyces cerevisiae. Flo11p is implicated in many developmental processes, including flocculation, formation of pseudohyphae, agar invasion, and formation of microbial mats and biofilms. However, Flo11p mediates different processes in different yeast strains. To investigate the mechanisms by which FLO11 determines these differences in colony morphology, flocculation, and invasion, we studied gene structure, function, and expression levels. Nonflocculent Saccharomyces cerevisiae Σ1278b cells exhibited significantly higher FLO11mRNA expression, especially in the stationary phase, than highly flocculent S. cerevisiae var. diastaticus. The two strains varied in cell surface hydrophobicity, and Flo11p contributed significantly to surface hydrophobicity in S. cerevisiae var. diastaticus but not in strain Σ1278b. Sequencing of the FLO11 gene in S. cerevisiae var. diastaticus revealed strain-specific differences, including a 15-amino-acid insertion in the adhesion domain. Flo11p adhesion domains from strain Σ1278b and S. cerevisiae var. diastaticus were expressed and used to coat magnetic beads. The adhesion domain from each strain bound preferentially to homologous cells, and the preferences were independent of the cells in which the adhesion domains were produced. These results are consistent with the idea that strain-specific variations in the amino acid sequences in the adhesion domains cause different Flo11p flocculation activities. The results also imply that strain-specific differences in expression levels, posttranslational modifications, and allelic differences outside the adhesion domains have little effect on flocculation.

Creative Commons License
Creative Commons Attribution 4.0
Citation Information
Li Li, Subit Barua, Peter N Lipke and Anne M Dranginis. "Molecular Basis for Strain Variation in the S. Cerevisiae adhesion Flo11p" MSphere Vol. 1 Iss. 4 (2016)
Available at: http://works.bepress.com/li-li3/10/