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Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae
Department of Plant Pathology: Faculty Publications
  • James E. Galagan, Broad Institute of MIT and Harvard
  • Sarah E. Calvo, Broad Institute of MIT and Harvard
  • Christina Cuomo, Broad Institute of MIT and Harvard
  • Li-Jun Ma, Broad Institute of MIT and Harvard
  • Jennifer R. Wortman, The Institute for Genomic Research, Rockville, Maryland
  • Serafim Batzoglou, Stanford University
  • Su-In Lee, Stanford University
  • Meray Baştürkmen, Oregon Health & Science University
  • Christina C. Spevak, Oregon Health & Science University
  • Vladimir Kapitonov, Genetic Information Research Institute, Mountain View, California
  • Jerzy Jurka, Genetic Information Research Institute, Mountain View, California
  • Claudio Scazzocchio, Université Paris-Sud , France
  • Mark Farman, University of Kentucky
  • Jonathan Butler, Broad Institute of MIT and Harvard
  • Seth Purcell, Broad Institute of MIT and Harvard
  • Steven D. Harris, University of Nebraska–Lincoln
  • Gerhard H. Braus, Georg-August-University Gottingen, Germany
  • Oliver Draht, Georg-August-University Gottingen, Germany
  • Silke Busch, Georg-August-University Gottingen, Germany
  • Christophe D'Enfert, Institut Pasteur, Paris, France
  • Christiane Bouchier, Institut Pasteur, Paris, France
  • Gustavo H. Goldman, Universidade de Sao Paulo, Brazil
  • Deborah Bell-Pedersen, Texas A&M University
  • Sam Griffiths-Jones, The Wellcome Trust Sanger Institute, Hinxton, UK
  • John H. Doonan, John Innes Centre, Norwich, UK
  • Jaehyuk Yu, University of Wisconsin-Madison
  • Kay Vienken, University of Karlsruhe, Germany
  • Arnab Pain, The Wellcome Trust Sanger Institute, Hinxton, UK
  • Michael Freitag, Oregon State University
  • Eric U. Selker, University of Oregon
  • David B. Archer, University of Nottingham, UK
  • Miguel Á. Peñalva, Consejo Superior de Investigaciones Científicas, Madrid, Spain
  • Berl R. Oakley, Ohio State University
  • Michelle Momany, University of Georgia
  • Toshihiro Tanaka, National Institute of Technology and Evaluation, Tokyo, Japan
  • Toshitaka Kumagai, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan
  • Kiyoshi Asai, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan
  • Masayuki Machida, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan
  • William C. Nierman, The Institute for Genomic Research, Rockville, Maryland
  • David W. Denning, University of Manchester, UK
  • Mark Caddick, University of Liverpool, UK
  • Michael Hynes, University of Melbourne, Australia
  • Mathieu Paoletti, University of Nottingham, UK
  • Reinhard Fischer, University of Karlsruhe, Germany
  • Bruce Miller, University of Idaho
  • Paul Dyer, University of Nottingham, UK
  • Matthew S. Sachs, Oregon Health & Science University
  • Stephen A. Osmani, Ohio State University
  • Bruce W. Birren, The Broad Institute of MIT and Harvard
Date of this Version
12-22-2005
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Published in Nature 438 (December 22, 2005), pp. 1105-1115; doi 10.1038/nature04341 Copyright © 2005 Nature Publishing Group. Used by permission.

Abstract

The aspergilli comprise a diverse group of filamentous fungi spanning over 200 million years of evolution. Here we report the genome sequence of the model organism Aspergillus nidulans, and a comparative study with Aspergillus fumigatus, a serious human pathogen, and Aspergillus oryzae, used in the production of sake, miso, and soy sauce. Our analysis of genome structure provided a quantitative evaluation of forces driving long-term eukaryotic genome evolution. It also led to an experimentally validated model of mating-type locus evolution, suggesting the potential for sexual reproduction in A. fumigatus and A. oryzae. Our analysis of sequence conservation revealed over 5,000 non-coding regions actively conserved across all three species. Within these regions, we identified potential functional elements including a previously uncharacterized TPP riboswitch and motifs suggesting regulation in filamentous fungi by Puf family genes. We further obtained comparative and experimental evidence indicating widespread translational regulation by upstream open reading frames. These results enhance our understanding of these widely studied fungi as well as provide new insight into eukaryotic genome evolution and gene regulation.

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Citation Information
James E. Galagan, Sarah E. Calvo, Christina Cuomo, Li-Jun Ma, et al.. "Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae" (2005)
Available at: http://works.bepress.com/li-jun_ma/1/