Skip to main content
Article
Determination of starch lysophospholipids in rice using liquid chromatography–mass spectrometry (LC-MS)
Journal of Agricultural and Food Chemistry
  • Lei Liu, Southern Cross University
  • Chuan Tong, Zhejiang University
  • Jinsong Bao, Zhejiang University
  • Daniel LE Waters, Southern Cross University
  • Terry J Rose, Southern Cross University
  • Graham J King, Southern Cross University
Document Type
Article
Publication Date
1-1-2014
Peer Reviewed
Peer-Reviewed
Abstract

Acquiring a complete understanding of rice starch lysophospholipids (LPLs), their biosynthetic pathways and genetic diversity, and the influence of genotype by environment interactions has been hampered by the lack of efficient high-throughput extraction and analysis methods. It was hypothesized a single-step aqueous n-propanol extraction combined with liquid chromatography–mass spectrometry (LC-MS) could be employed to analyze starch LPLs in white rice. The investigation found different grinding methods showed little effect on the final LPL detected, and a simple single-step extraction with 75% n-propanol (8 mL/0.15 g) heated at 100 °C for 2 h was as effective as an onerous multistep extraction method. A LC-MS method was optimized to simultaneously quantify 10 major LPLs in rice starch within 15 min. This method enables total and individual starch LPL analysis of a large number of rice samples at little cost. This approach could be applied to starch LPLs in other cereals.

Disciplines
Citation Information

Liu, L, Tong, C, Bao, J, Waters, DL, Rose, TJ & King, GJ 2014, 'Determination of starch lysophospholipids in rice using liquid chromatography–mass spectrometry (LC-MS)', Journal of Agricultural and Food Chemistry, vol. 62, no. 28, pp. 6600-7.

Published version available from:

http://doi.org/10.1021/jf500585j