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Comparative proteomic analyses of human adipose extracellular matrices decellularized using alternative procedures
Journal of Biomedical Materials Research Part A
  • Caasy Porch-Thomas, Biomedical Science Program, Tulane University School of Medicine & Center for Stem Cell Research & Regenerative Medicine, Tulane University School of Medicine
  • Jie Li, Center for Stem Cell Research & Regenerative Medicine, Tulane University School of Medicine & National Engineering Laboratory for Oral Regenerative Medicine, West China School of Stomatology, Sichuan University
  • Fabiana Zanata, Center for Stem Cell Research & Regenerative Medicine, Tulane University School of Medicine & Federal University of Sao Paulo
  • Elizabeth C Martin, Department of Biological and Agricultural Engineering, Louisiana State University
  • Nicholas Pashos, Center for Stem Cell Research & Regenerative Medicine, Tulane University School of Medicine
  • Kaylynn Genemaras, Center for Stem Cell Research & Regenerative Medicine, Tulane University School of Medicine
  • Nicholas J Poche, Department of Biological and Agricultural Engineering, Louisiana State University
  • Nicholas P Totaro, Department of Biological and Agricultural Engineering, Louisiana State University
  • Melyssa R Bratton, Department of Chemistry, Xavier University of Louisiana
  • Dina Gaupp, Center for Stem Cell Research & Regenerative Medicine, Tulane University School of Medicine
  • Trivia Frazier, Center for Stem Cell Research & Regenerative Medicine, Department of Structural and Cell Biology, , Tulane University School of Medicine & LaCell LLC, New Orleans, Louisiana
  • Xiying Wu, LaCell LLC, New Orleans, Louisiana
  • Lydia Masako Ferreira, Federal University of Sao Paulo
  • Weidong Tian, National Engineering Laboratory for Oral Regenerative Medicine, West China School of Stomatology, Sichuan University
  • Guangdi Wang, Department of Chemistry, Xavier University of Louisiana
  • Bruce A Bunnell, Center for Stem Cell Research & Regenerative Medicine, Department of Pharmacology, Tulane University School of Medicine
  • Lauren Flynn, Department of Chemical and Biochemical Engineering, Department of Anatomy and Cell Biology, Western University
  • Daniel Hayes, Department of Biomedical Engineering, Pennsylvania State University
  • Jeffrey M Gimble, Department of Surgery, Tulane University School of Medicine
Document Type
Article
Publication Date
9-1-2018
URL with Digital Object Identifier
https://doi.org/10.1002/jbm.a.36444
Abstract

Decellularized human adipose tissue has potential clinical utility as a processed biological scaffold for soft tissue cosmesis, grafting, and reconstruction. Adipose tissue decellularization has been accomplished using enzymatic-, detergent-, and/or solvent-based methods. To examine the hypothesis that distinct decellularization processes may yield scaffolds with differing compositions, the current study employed mass spectrometry to compare the proteomes of human adipose-derived matrices generated through three independent methods combining enzymatic-, detergent-, and/or solvent-based steps. In addition to protein content, bioscaffolds were evaluated for deoxyribose nucleic acid depletion, extracellular matrix composition, and physical structure using optical density, histochemical staining, and scanning electron microscopy. Mass spectrometry based proteomic analyses identified 25 proteins (having at least two peptide sequences detected) in the scaffolds generated with an enzymatic approach, 143 with the detergent approach, and 102 with the solvent approach, as compared to 155 detected in unprocessed native human fat. Immunohistochemical detection confirmed the presence of the structural proteins actin, collagen type VI, fibrillin, laminin, and vimentin. Subsequent in vivo analysis of the predominantly enzymatic- and detergent-based decellularized scaffolds following subcutaneous implantation in GFP(+) transgenic mice demonstrated that the matrices generated with both approaches supported the ingrowth of host-derived adipocyte progenitors and vasculature in a time dependent manner. Together, these results determine that decellularization methods influence the protein composition of adipose tissue-derived bioscaffolds. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A:2481-2493, 2018.

Notes

This is an author-accepted manuscript. The final published version is published at: J Biomed Mater Res A. 2018 September ; 106(9): 2481–2493. doi:10.1002/jbm.a.36444

Citation Information
Caasy Porch-Thomas, Jie Li, Fabiana Zanata, Elizabeth C Martin, et al.. "Comparative proteomic analyses of human adipose extracellular matrices decellularized using alternative procedures" Journal of Biomedical Materials Research Part A Vol. 106 Iss. 9 (2018) p. 2481 - 2493
Available at: http://works.bepress.com/lauren-flynn/5/