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Article
Tocotrienol Vitamin E Protects against Preclinical Canine Ischemic Stroke by Inducing Arteriogenesis
Journal of Cerebral Blood Flow and Metabolism
  • Cameron Rink
  • Greg Christoforidis
  • Savita Khanna
  • Laura Peterson, Wright State University
  • Yojan Patel
  • Suchin Khanna
  • Amir Abduljalil
  • Okan Irfanoglu
  • Raghu Machiraju
  • Valerie K. Bergdall
  • Chandan K. Sen
Document Type
Article
Publication Date
11-1-2011
Abstract
Vitamin E consists of tocopherols and tocotrienols, in which α-tocotrienol is the most potent neuroprotective form that is also effective in protecting against stroke in rodents. As neuroprotective agents alone are insufficient to protect against stroke, we sought to test the effects of tocotrienol on the cerebrovascular circulation during ischemic stroke using a preclinical model that enables fluoroscopy-guided angiography. Mongrel canines (mean weight=26.3±3.2 kg) were supplemented with tocotrienol-enriched (TE) supplement (200 mg b.i.d, n=11) or vehicle placebo (n=9) for 10 weeks before inducing transient middle cerebral artery (MCA) occlusion. Magnetic resonance imaging was performed 1 hour and 24 hours post reperfusion to assess stroke-induced lesion volume. Tocotrienol-enriched supplementation significantly attenuated ischemic stroke-induced lesion volume (P<0.005). Furthermore, TE prevented loss of white matter fiber tract connectivity after stroke as evident by probabilistic tractography. Post hoc analysis of cerebral angiograms during MCA occlusion revealed that TE-supplemented canines had improved cerebrovascular collateral circulation to the ischemic MCA territory (P<0.05). Tocotrienol-enriched supplementation induced arteriogenic tissue inhibitor of metalloprotease 1 and subsequently attenuated the activity of matrix metalloproteinase-2. Outcomes of the current preclinical trial set the stage for a clinical trial testing the effects of TE in patients who have suffered from transient ischemic attack and are therefore at a high risk for stroke.
DOI
10.1038/jcbfm.2011.85
Citation Information
Cameron Rink, Greg Christoforidis, Savita Khanna, Laura Peterson, et al.. "Tocotrienol Vitamin E Protects against Preclinical Canine Ischemic Stroke by Inducing Arteriogenesis" Journal of Cerebral Blood Flow and Metabolism Vol. 31 Iss. 11 (2011) p. 2218 - 2230 ISSN: 0271678X
Available at: http://works.bepress.com/laura_peterson/14/