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Article
Time of Day Regulates Subcellular Trafficking, Tripartite Synaptic Localization, and Polyadenylation of the Astrocytic Fabp7 mRNA
The Journal of Neuroscience
  • Jason R. Gerstner
  • William M. Vanderheyden
  • Timothy LaVaute
  • Cara J. Westmark
  • Labib Rouhana, Wright State University - Main Campus
  • Allan I. Pack
  • Marvin Wickens
  • Charles F. Landry
Document Type
Article
Publication Date
1-25-2012
Abstract

The astrocyte brain fatty acid binding protein (Fabp7) has previously been shown to have a coordinated diurnal regulation of mRNA and protein throughout mouse brain, and an age-dependent decline in protein expression within synaptoneurosomal fractions. Mechanisms that control time-of-day changes in expression and trafficking Fabp7 to the perisynaptic process are not known. In this study, we confirmed an enrichment of Fabp7 mRNA and protein in the astrocytic perisynaptic compartment, and observed a diurnal change in the intracellular distribution of Fabp7 mRNA in molecular layers of hippocampus. Northern blotting revealed a coordinated time-of-day-dependent oscillation for the Fabp7 mRNA poly(A) tail throughout murine brain. Cytoplasmic polyadenylation element-binding protein 1 (CPEB1) regulates subcellular trafficking and translation of synaptic plasticity-related mRNAs. Here we show that Fabp7 mRNA coimmunoprecipitated with CPEB1 from primary mouse astrocyte extracts, and its 3′UTR contains phylogenetically conserved cytoplasmic polyadenylation elements (CPEs) capable of regulating translation of reporter mRNAs during Xenopus oocyte maturation. Given that Fabp7 expression is confined to astrocytes and neural progenitors in adult mouse brain, the synchronized cycling pattern of Fabp7 mRNA is a novel discovery among known CPE-regulated transcripts. These results implicate circadian, sleep, and/or metabolic control of CPEB-mediated subcellular trafficking and localized translation of Fabp7 mRNA in the tripartite synapse of mammalian brain.

Comments

Copyright © 2012 the authors. Final published version is also available via https://doi.org/10.1523/JNEUROSCI.3228-11.2012.

DOI
10.1523/JNEUROSCI.3228-11.2012
Citation Information
Jason R. Gerstner, William M. Vanderheyden, Timothy LaVaute, Cara J. Westmark, et al.. "Time of Day Regulates Subcellular Trafficking, Tripartite Synaptic Localization, and Polyadenylation of the Astrocytic Fabp7 mRNA" The Journal of Neuroscience Vol. 32 Iss. 4 (2012) p. 1383 - 1394 ISSN: 02706474
Available at: http://works.bepress.com/labib_rouhana/4/