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Article
Inheritance of a Nuclear PIWI from Pluripotent Stem Cells by Somatic Descendants Ensures Differentiation by Silencing Transposons in Planarian
Developmental Cell
  • Norito Shibata
  • Makoto Kashima
  • Taisuke Ishiko
  • Osamu Nishimura
  • Labib Rouhana, Wright State University - Main Campus
  • Kazuyo Misaki
  • Shigenobu Yonemura
  • Kuniaki Saito
  • Haruhiko Siomi
  • Mikiko C. Siomi
  • Kiyokazu Agata
Document Type
Article
Publication Date
5-9-2016
Abstract
Differentiation of pluripotent stem cells (PSCs) requires transposon silencing throughout the process. PIWIs, best known as key factors in germline transposon silencing, are also known to act in somatic differentiation of planarian PSCs (neoblasts). However, how PIWIs control the latter process remains elusive. Here, using Dugesia japonica, we show that a nuclear PIWI, DjPiwiB, was bound to PIWI-interacting RNAs (generally key mediators of PIWI-dependent transposon silencing), and was detected in not only neoblasts but also their descendant somatic cells, which do not express piwi. In contrast, cytoplasmic DjPiwiA and DjPiwiC were detected only in neoblasts, in accord with their transcription there. DjPiwiB was indispensable for regeneration, but dispensable for transposon silencing in neoblasts. However, transposons were derepressed at the onset of differentiation in DjPiwiB-knockdown planarians. Thus, DjPiwiB appears to be inherited by descendant somatic cells of neoblasts to ensure transposon silencing in those cells, which are unable to produce PIWI proteins.
DOI
10.1016/j.devcel.2016.04.009
Citation Information
Norito Shibata, Makoto Kashima, Taisuke Ishiko, Osamu Nishimura, et al.. "Inheritance of a Nuclear PIWI from Pluripotent Stem Cells by Somatic Descendants Ensures Differentiation by Silencing Transposons in Planarian" Developmental Cell Vol. 37 Iss. 3 (2016) p. 226 - 237 ISSN: 15345807
Available at: http://works.bepress.com/labib_rouhana/18/