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Contribution to Book
Analysis of Stem Cell Motility In Vivo Based on Immunodetection of Planarian Neoblasts and Tracing of BrdU-Labeled Cells After Partial Irradiation
Cytoskeleton Methods and Protocols
  • Junichi Tasaki, Wright State University - Main Campus
  • Chihiro Uchiyama-Tasaki
  • Labib Rouhana, Wright State University - Main Campus
Document Type
Book Chapter
Publication Date
1-1-2016
Abstract
Planarian flatworms have become an important system for the study of stem cell behavior and regulation in vivo. These organisms are able to regenerate any part of their body upon damage or amputation. A crucial cellular event in the process of planarian regeneration is the migration of pluripotent stem cells (known as neoblasts) to the site of injury. Here we describe two approaches for analyzing migration of planarian stem cells to an area where these have been ablated by localized X-ray irradiation. The first approach involves immunolabeling of mitotic neoblasts, while the second is based on tracing stem cells and their progeny after BrdU incorporation. The use of planarians in studies of cell motility is suitable for the identification of factors that influence stem cell migration in vivo and is amenable to RNA interference or pharmacological screening.
DOI
10.1007/978-1-4939-3124-8_18
Citation Information
Junichi Tasaki, Chihiro Uchiyama-Tasaki and Labib Rouhana. "Analysis of Stem Cell Motility In Vivo Based on Immunodetection of Planarian Neoblasts and Tracing of BrdU-Labeled Cells After Partial Irradiation" Cytoskeleton Methods and Protocols Vol. 1365 (2016) p. 323 - 338 ISSN: 9781493931231
Available at: http://works.bepress.com/labib_rouhana/13/